英语文档的作业

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1、ConstructionDNAfingerprintingofIxerispolycephalavarieties(lines)baesdonEST-SSRandSRAPmarkersBANQian1,HUANGLin-kai1,ZHANGXin-quan1*,(DepartmentofGrasslandScience,SichuanAgriculturalUniversity,Chengdu611130,China)Abstract:Ixerispolycephalaisexcellentgrass-fodderannualAsteraceaeforage

2、WiththeintentionofconstmctionDNAfingerprintingamong14Ixerispolycephalavarieties(lines),twentyfourof152EST-SSRprimersand32of209SRAPprimerswerescreenedaccordingtoclearbandsandhighpolymorphicimfbrmation.Totalof270bandsincluding223polymorphicbandshadbeenamplifiedfrom24EST-SSRprimers,th

3、epolymorphisminformationcontentvaluesreached0.834.TheNei'sgenediversity(H)valuesrangedfrom0.2464to0.4288,andtheShannon'sinformationindex(/)valuesrangedfrom0.3597to0.6148,thedistinguishedvarieties(lines)numberrangedfrom3to14.ThirtytwoSRAPprimersdetected367polymorphicbandsandtheavera

4、gevalueofpolymorphisminformationcontentwas0.826.TheNei'sgenediversity(H)valuesrangedfrom0.2006to0.389&andtheShannon'sinformationindex(/)valuesrangedfrom0.3167to0.5716,thedistinguishedvarieties(lines)numberrangedfrom2to12.Ninevarieties(lines)emergedspecialbandson7primers,andtookcomp

5、rehensivelyconsiderationofeachgeneticdiversityindex,obtainedatotalof40polymorphicbandsfrom5coreprimers(IpSSR102、IpSSR8()^IpSSR91、Mel0+Em5>Me9+Eml7)forDNAfingerprintingconstructionof14Ixerispolycephalavarieties(lines),andeachvarieties(lines)hadamolecularstandardDNAfingerprintingcode

6、・KeyWords:Ixreispolycephalavariety(line);EST-SSRmarker;SRAPmarker;Coreprimer;DNAfingerprintingINTRODUCTIONMATERIALSandMETHODSPlantmaterialsAtotalof30sampleswereusedinthisstudy(Table1).MostofnewlineswerefromForageResearchCenterinSichuanAgriculturalUniversityandwereoriginallycollecte

7、din2000-2010byformerbreeders・Othercultivarsandwildgermplasmswerecollectedatlocalmarketoverlast10years.TheseedsofeachcultivarweregenninatedonabsorbentfilterpaperinPetridishesatatemperatureof20°Cafterapretreatmentatatemperatureof3°C・Germinatedseedsweretransplantedintoasand-peatdmixtu

8、re,andmaintainedinagreenho