密歇根大学生物系实验室的常用试剂配方

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1、TableofContentsLBMedium.............................................................................................1NZMedium............................................................................................2SMBuffer.....................................................................

2、...........................3SETBuffer...............................................................................................46XPrehybSoln.......................................................................................510XTBE........................................................

3、...........................................610XTAE..................................................................................................720XSSC...................................................................................................81%SDS,0.2MNaOH..........................

4、..................................................914%PEG(8000),2MNaCl,10mMMgSO4.....................................1020%SDS.................................................................................................111.0MTris,pH8.0,1.5MNaCl................................................

5、...........1210mMTris-HCl,pH7.5,10mMMgSO4...........................................1310mMTris,50mMEDTA,pH7.5....................................................1410mMTris-HCl,1mMEDTA,pH7.5.............................................153MSodiumAcetate,pH4.8......................................

6、.........................16Electrophoresisdye...............................................................................17LabellingStopdye.................................................................................18Sequencinggeldye......................................................

7、.........................195%Acrylamide......................................................................................206%AcrylamideinTBE,50%Urea.....................................................2140%Acrylamide..........