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1、XX大学毕业论文麦冬水煎剂的遗传毒性研究姓名：2014年6月25日麦冬水煎剂的遗传毒性研究作者：胡燕平，宋捷，王欣，张旻，王秀文，李波【摘要】目的采用小鼠淋巴瘤细胞试验(MLA)和小鼠骨髓微核试验(MNT)研究麦冬水煎剂的遗传毒性。方法在MLA中,3.36、6.73、13.45、26.90mg(原药材)/mL4个浓度组在非代谢活化(・S9)和代谢活化什S9)条件下与L5178Y细胞作用3h,表达2d,制备突变频率平板并培养12d,计数大、小细胞集落的孔数，计算总突变率(MF)和小集落突变'B■分率(SC
2、%)；在MNT中，每组10只ICR小鼠，雌雄各半,13.35、6.68、3.34g(原药材)/kg3个剂量组间隔24h灌胃给药2次，制作骨髓涂片，计数每只小鼠2000个嗜多染红细胞屮含微核的嗜多染红细胞数,计算每组动物嗜多染红细胞微核率的平均值。结果MLA4个浓度组在+/・S9条件下诱发的MF呈现剂量相关性增加，与阴性对照组比较有统计学意义(PV0.01),SC%与阴性对照组相仿；MNT各剂量组未显示骨髓抑制作用,诱发的微核率与阴性对照组比较未见明显增加。结论麦冬水煎剂在+/-S9条件下均口J诱发L51
3、78Y细胞tk位点突变,提示其可能存在诱变物质；但该供试品对小鼠骨髓细胞染色体无损伤,经体内代谢活化后未显示遗传毒性作用。【关键词】麦冬水煎剂；淋巴瘤细胞试验；骨髓微核试验；遗传毒性；小鼠Abstract:ObjectiveToinvestigategenotoxicityofRadixOphiopogonisdecoctionbyMouseLymphomaAssay(MLA)andMouseBoneMarrowMicronucleusTest(MNT).MethodsInMLA,fourdoselev
4、elsof3.36,6.73,13.45,26.90mg(crudedrug)/mLwasexposedwithL5178Ycellsfor3hourswithandwithoutmetabolicactivation(+/-S9),thenexpressedfor2days.Themutationfrequencyplateswerepreparedandincubatedfor12days.Colonysizeineachplatewasscored,andthetotalmutationfrequ
5、encyandthepercentageofsmallcolonymutantswerecalculatedandanalyzed.InMNT,containedthreegroupsof3.34,6.6&13.35g(crudedrug)/kgand10ICRmice(5males/5females)ineachgroup,themiceweregivenevery24hoursbyoralgavagetwiceandsacrificedafter24hoursofthelastdosing,both
6、femurboneswerecollectedtopreparethesmea匚Foreachmouse,thenumberofmicronucleatedpolychromaticerythrocytes(MNPCE)in2000polychromaticerythrocyteswascounted,andthemeanrateofMNPCEineachgroupwascalculated.ResultsInMLA,thetotalmutationfrequencyofdoselevelsshowed
7、adose-dependentincrease,therewasstatisticalsignificant(P<0.01)comparedwithnegativecontrol,andthepercentageofsmallcolonymutantswassimilarwithnegativecontrolintheabsenceandthepresenceS9・InMNT,thedecoctiondidnotshowinhibitorycfficctforbonemarrow,andtherateo
8、fMNPCEinducedineachgroupdidnotshowsignificantincreasecomparingwithnegativecontrol.ConclusionRadixOphiopogonisdecoctioncaninducetkgenemutationinL5178Ycellswithandwithoutmetabolicactivation,ithintsthatthetestarticlemayhavepo
