miR-18a调节HTR-8细胞中雌激素受体α表达的研究

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1、miR-18a调节HTR-8细胞中雌激素受体a表达的研究杨洋徐妍妍马媛西安医学院第一附属医院第四军医大学唐都医院摘要：目的:研究微小RNA-18a(miR-18a)在人正常滋养细胞(IITR8)屮对雌激素受体a(ERa)表达的调控作用。方法:miR-18a前体分子［miR-18a类似物(miR-18amimics)>miR-18a空白载体>miR~18a抑制物(miR-18ainhibitor)］转染HTR-8细胞，分为实验组1、阴性对照组(NC组)、实验组2。RT-PCR检测转染后各组中miR-18a的表达情况;RT-PCR与Western-blot检

2、测HTR-8细胞中ERa在mRNA和蛋白水平的表达情况。结果:实验组1细胞屮miR-18a的表达与NC组(0.407±0.019)相比明显增高(0.880±0.060),实验组2细胞中miR-18a的表达显著降低(0.160±0.014),差异有统计学意义(P<0.05)。转染48小时后实验组1ERamRNA的表达(0.249±0.003)与NC组(0.313±0.010)相比差异无统计学意义(P>0.05);实验组2ERamRNA的表达水平明显增高(0.823±0.023),差异有统计学意义(P<0.05)。实验组2ERa蛋白表达水平(1.030±0.

3、006)与NC组(0.960±0.008)相比有增高，实验组1ERa蛋白表达水平明显降低(0.660±0.013),差异有统计学意义(IK0.05)。结论:在人正常滋养细胞中，miR-18a可能在转录和翻译水平均对ERa具有调控作用。关键词：微小R7A;滋养细胞;雌激素受体;子痫前期;作者简介：马媛，E-ma订:361577992@qq.com收稿日期：2017-02-22EffectofPERelatedmiR-18aonExpressionofEstrogenReceptorAlphainHTR-8CellsYANGYangXUYanvanMAYua

4、nTheFirstAffiliatedHospitalofXianMedicalUniversity;TongDuHospitalofFourthMilitaryMedicalUniversity;Abstract：Objective:TostudytheeffectofmiR-18aonexpressionofestrogenreceptoralphainhumannormaltrophoblastcel1s(HTR-8).Methods:ThesynthcsizcdmiR-18a(miR-18amimics,miR-18ainhibitorandbl

5、ankcarrier)weretransfectedintoHTR-8cells.Experimentincludedexperimentalgroup1,pre-miRNAun-transfectiongroup(NCgroup)andexperimentalgroup3.RT-PCRdetectedtheexpressionofmiR-18ainthethreegroups.RT-PCRandWestern-blotdetectedtheexpressionofERamRNAandproteininthetransfccted11TR-8cells.

6、RcsuIts:AccordingtotheresuItsofRT-PCR,theexpressionsofmiR-18ainexperimentalgroup1(0.880±0.060)wassignificantlyhigherthanthatofNCgroup(0.407±0.019)(P<0.05),andtheexpressionsofmiR-18ainexperimentalgroup3(0.160+0.014)wassignificantly1owerthonthatofNCgroup(0.407±0.019)(P<0.05);Aftert

7、remsfcctcdfor48hours,therewasnosignifiedntdifferencebetweentheexpressionofERamRNAbetweenexperimentalgroup1(0.249±0.003)andNCgroup(0.313±0.010)(P>0.05),theexpressionofERamRNAintransfectedcellsinexperimentalgroup3(0.823土0.023)wassignifiedntlyhigherthanthatofNCgroup(P<0.05);Western-

8、blotresuItsshowedthattheexpressionofERap