保护碱基添加总结

《保护碱基添加总结》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、酶切位点保护碱基表6酶寡核苷酸序列切割率%2hr20hrPvuICCGATCGGATCGATCGATTCGCGATCGCGA010002510SacICGAGCTCG1010SacIIGCCGCGGCTCCCCGCGGGGA0500>90SalIGTCGACGTCAAAAGGCCATAGCGGCCGCGCGTCGACGTCTTGGCCATAGCGGCCGCGGACGCGTCGACGTCGGCCATAGCGGCCGCGGAA0101005075ScaIGAGTACTCAAAAGTACTTTT10752575SmaICCCGGGCCC

2、CGGGGCCCCCGGGGGTCCCCCGGGGGA0010>90101050>90SpeIGACTAGTCGGACTAGTCCCGGACTAGTCCGCTAGACTAGTCTAG101000>90>905050酶切位点保护碱基表2关键词：酶切位点保护碱基表2                                        酶寡核苷酸序列切割率%2hr20hrNotITTGCGGCCGCAAATTTGCGGCCGCTTTAAAATATGCGGCCGCTATAAAATAAGAATGCGGCCGCTAAACTATAAG

3、GAAAAAAGCGGCCGCAAAAGGAAAA0101025250101090>90NsiITGCATGCATGCACCAATGCATTGGTTCTGCAGTT10>90>90>90PacITTAATTAAGTTAATTAACCCTTAATTAAGG000025>90PmeIGTTTAAACGGTTTAAACCGGGTTTAAACCCAGCTTTGTTTAAACGGCGCGCCGG0007502550>90PstIGCTGCAGCTGCACTGCAGTGCAAACTGCAGAACCAATGCATTGGAAAACTGCAGCCA

4、ATGCATTGGAACTGCAGAACCAATGCATTGGATGCAT010>90>900010>90>900PvuICCGATCGGATCGATCGATTCGCGATCGCGA010002510SacICGAGCTCG1010SacIIGCCGCGGCTCCCCGCGGGGA0500>90SalIGTCGACGTCAAAAGGCCATAGCGGCCGCGCGTCGACGTCTTGGCCATAGCGGCCGCGGACGCGTCGACGTCGGCCATAGCGGCCGCGGAA0101005075ScaIGAGTACTCAAAA

5、GTACTTTT10752575SmaICCCGGGCCCCGGGGCCCCCGGGGGTCCCCCGGGGGA0010>90101050>90SpeIGACTAGTCGGACTAGTCCCGGACTAGTCCGCTAGACTAGTCTAG101000>90>905050SphIGGCATGCCCATGCATGCATGACATGCATGCATGT001002550StuIAAGGCCTTGAAGGCCTTCAAAAGGCCTTTT>90>90>90>90>90>90XbaICTCTAGAGGCTCTAGAGCTGCTCTAGAGC

6、ACTAGTCTAGACTAG0>9075750>90>90>90XhoICCTCGAGGCCCTCGAGGGCCGCTCGAGCGG0101002575XmaICCCCGGGGCCCCCGGGGGCCCCCCGGGGGGTCCCCCCGGGGGGA02550>90075>90>90酶寡核苷酸序列切割率%2hr20hrAccIGGTCGACCCGGTCGACCGCCGGTCGACCGG000000AflIIICACATGTGCCACATGTGGCCCACATGTGGG0>90>900>90>90AscIGGCGCGCCAGGCGC

7、GCCTTTGGCGCGCCAA>90>90>90>90>90>90AvaICCCCGGGGCCCCCGGGGGTCCCCCGGGGGA50>90>90>90>90>90BamHICGGATCCGCGGGATCCCGCGCGGATCCGCG10>90>9025>90>90BglIICAGATCTGGAAGATCTTCGGAAGATCTTCC075250>90>90BssHIIGGCGCGCCAGGCGCGCCTTTGGCGCGCCAA005000>90BstEIIGGGT(A/T)ACCC010BstXIAACTGCAGAACCA

8、ATGCATTGGAAAACTGCAGCCAATGCATTGGAACTGCAGAACCAATGCATTGGATGCAT02525050>90ClaICATCGATGGATCGATCCCATCGATGGCCCATCGATGGG00>905000>90