neb酶切位点保护碱基

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1、PCR设计引物时酶切位点的保护切割率%酶寡核苷酸序列2hr20hrAccIGGTCGACC00CGGTCGACCG00CCGGTCGACCGG00AflIIICACATGTG00CCACATGTGG>90>90CCCACATGTGGG>90>90AscIGGCGCGCC>90>90AGGCGCGCCT>90>90TTGGCGCGCCAA>90>90AvaICCCCGGGG50>90CCCCCGGGGG>90>90TCCCCCGGGGGA>90>90BamHICGGATCCG1025CGGGATCCCG>90>90CGCGGATCCGCG>90>90BglIICAGATCTG00G

2、AAGATCTTC75>90GGAAGATCTTCC25>90BssHIIGGCGCGCC00AGGCGCGCCT00TTGGCGCGCCAA50>90BstEIIGGGT(A/T)ACCC010BstXIAACTGCAGAACCAATGCATTGG00AAAACTGCAGCCAATGCATTGGAA2550CTGCAGAACCAATGCATTGGATGCAT25>90ClaICATCGATG00GATCGATC00CCATCGATGG>90>90CCCATCGATGGG5050EcoRIGGAATTCC>90>90CGGAATTCCG>90>90CCGGAATTCCGG>90>

3、90HaeIIIGGGGCCCC>90>90AGCGGCCGCT>90>90TTGCGGCCGCAA>90>90HindIIICAAGCTTG00CCAAGCTTGG00CCCAAGCTTGGG1075KpnIGGGTACCC00GGGGTACCCC>90>90CGGGGTACCCCG>90>90MluIGACGCGTC00CGACGCGTCG2550NcoICCCATGGG00CATGCCATGGCATG5075NdeICCATATGG00CCCATATGGG00CGCCATATGGCG00GGGTTTCATATGAAACCC00GGAATTCCATATGGAATTCC75>9

4、0GGGAATTCCATATGGAATTCCC75>90NheIGGCTAGCC00CGGCTAGCCG1025CTAGCTAGCTAG1050NotITTGCGGCCGCAA00ATTTGCGGCCGCTTTA1010AAATATGCGGCCGCTATAAA1010ATAAGAATGCGGCCGCTAAACTAT2590AAGGAAAAAAGCGGCCGCAAAAGGAAAA25>90NsiITGCATGCATGCA10>90CCAATGCATTGGTTCTGCAGTT>90>90PacITTAATTAA00GTTAATTAAC025CCTTAATTAAGG0>90PmeIGT

5、TTAAAC00GGTTTAAACC025GGGTTTAAACCC050AGCTTTGTTTAAACGGCGCGCCGG75>90PstIGCTGCAGC00TGCACTGCAGTGCA1010AACTGCAGAACCAATGCATTGG>90>90AAAACTGCAGCCAATGCATTGGAA>90>90CTGCAGAACCAATGCATTGGATGCAT00PvuICCGATCGG00ATCGATCGAT1025TCGCGATCGCGA010SacICGAGCTCG1010SacIIGCCGCGGC00TCCCCGCGGGGA50>90SalIGTCGACGTCAAAAGG

6、CCATAGCGGCCGC00GCGTCGACGTCTTGGCCATAGCGGCCGCG1050G1075ACGCGTCGACGTCGGCCATAGCGGCCGCGGAAScaIGAGTACTC1025AAAAGTACTTTT7575SmaICCCGGG010CCCCGGGG010CCCCCGGGGG1050TCCCCCGGGGGA>90>90SpeIGACTAGTC10>90GGACTAGTCC10>90CGGACTAGTCCG050CTAGACTAGTCTAG050SphIGGCATGCC00CATGCATGCATG025ACATGCATGCATGT1050StuIAAGGC

7、CTT>90>90GAAGGCCTTC>90>90AAAAGGCCTTTT>90>90XbaICTCTAGAG00GCTCTAGAGC>90>90TGCTCTAGAGCA75>90CTAGTCTAGACTAG75>90XhoICCTCGAGG00CCCTCGAGGG1025CCGCTCGAGCGG1075XmaICCCCGGGG00CCCCCGGGGG2575CCCCCCGGGGGG50>90TCCCCCCGGGGGGA>90>90注释：1．如果要加在序列的5‘端，就在酶切位点识