常用酶切位点表(含保护碱基)

《常用酶切位点表(含保护碱基)》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。

1、酶寡核苷酸序列切割率%2hr20hrAccIGGTCGACCCGGTCGACCGCCGGTCGACCGG000000AflIIICACATGTGCCACATGTGGCCCACATGTGGG0>90>900>90>90AscIGGCGCGCCAGGCGCGCCTTTGGCGCGCCAA>90>90>90>90>90>90AvaICCCCGGGGCCCCCGGGGGTCCCCCGGGGGA50>90>90>90>90>90BamHICGGATCCGCGGGATCCCGCGCGGATCCGCG10>90>9025>90>90BglIICAGATCT

2、GGAAGATCTTCGGAAGATCTTCC075250>90>90BssHIIGGCGCGCCAGGCGCGCCTTTGGCGCGCCAA005000>90BstEIIGGGT(A/T)ACCC010BstXIAACTGCAGAACCAATGCATTGGAAAACTGCAGCCAATGCATTGGAACTGCAGAACCAATGCATTGGATGCAT02525050>90ClaICATCGATGGATCGATCCCATCGATGGCCCATCGATGGG00>905000>9050EcoRIGGAATTCCCGGAATTCCGCCGGA

3、ATTCCGG>90>90>90>90>90>90HaeIIIGGGGCCCCAGCGGCCGCTTTGCGGCCGCAA>90>90>90>90>90>90HindIIICAAGCTTGCCAAGCTTGGCCCAAGCTTGGG00100075KpnIGGGTACCCGGGGTACCCCCGGGGTACCCCG0>90>900>90>90MluIGACGCGTCCGACGCGTCG025050NcoICCCATGGGCATGCCATGGCATG050075NdeICCATATGGCCCATATGGGCGCCATATGGCGGGGTTTCA

4、TATGAAACCCGGAATTCCATATGGAATTCCGGGAATTCCATATGGAATTCCC000075750000>90>90NheIGGCTAGCCCGGCTAGCCGCTAGCTAGCTAG0101002550酶寡核苷酸序列切割率%2hr20hrNotITTGCGGCCGCAAATTTGCGGCCGCTTTAAAATATGCGGCCGCTATAAAATAAGAATGCGGCCGCTAAACTATAAGGAAAAAAGCGGCCGCAAAAGGAAAA0101025250101090>90NsiITGCATGCATGCACCA

5、ATGCATTGGTTCTGCAGTT10>90>90>90PacITTAATTAAGTTAATTAACCCTTAATTAAGG000025>90PmeIGTTTAAACGGTTTAAACCGGGTTTAAACCCAGCTTTGTTTAAACGGCGCGCCGG0007502550>90PstIGCTGCAGCTGCACTGCAGTGCAAACTGCAGAACCAATGCATTGGAAAACTGCAGCCAATGCATTGGAACTGCAGAACCAATGCATTGGATGCAT010>90>900010>90>900PvuICCGATCGG

6、ATCGATCGATTCGCGATCGCGA010002510SacICGAGCTCG1010SacIIGCCGCGGCTCCCCGCGGGGA0500>90SalIGTCGACGTCAAAAGGCCATAGCGGCCGCGCGTCGACGTCTTGGCCATAGCGGCCGCGGACGCGTCGACGTCGGCCATAGCGGCCGCGGAA0101005075ScaIGAGTACTCAAAAGTACTTTT10752575SmaICCCGGGCCCCGGGGCCCCCGGGGGTCCCCCGGGGGA0010>90101050>90Spe

7、IGACTAGTCGGACTAGTCCCGGACTAGTCCGCTAGACTAGTCTAG101000>90>905050SphIGGCATGCCCATGCATGCATGACATGCATGCATGT001002550StuIAAGGCCTTGAAGGCCTTCAAAAGGCCTTTT>90>90>90>90>90>90XbaICTCTAGAGGCTCTAGAGCTGCTCTAGAGCACTAGTCTAGACTAG0>9075750>90>90>90XhoICCTCGAGGCCCTCGAGGGCCGCTCGAGCGG0101002575XmaI

8、CCCCGGGGCCCCCGGGGGCCCCCCGGGGGGTCCCCCCGGGGGGA02550>90075>90>90