保护碱基列表.doc

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1、.PCR设计引物时酶切位点的保护酶寡核苷酸序列切割率%2hr20hrAccIGGTCGACCCGGTCGACCGCCGGTCGACCGG000000AflIIICACATGTGCCACATGTGGCCCACATGTGGG0>90>900>90>90AscIGGCGCGCCAGGCGCGCCTTTGGCGCGCCAA>90>90>90>90>90>90AvaICCCCGGGGCCCCCGGGGGTCCCCCGGGGGA50>90>90>90>90>90BamHICGGATCCGCGGGATCCCGCGCGGATCCGCG10>90>9025>90>90Bg

2、lIICAGATCTGGAAGATCTTCGGAAGATCTTCC075250>90>90BssHIIGGCGCGCCAGGCGCGCCTTTGGCGCGCCAA005000>90BstEIIGGGT(A/T)ACCC010BstXIAACTGCAGAACCAATGCATTGGAAAACTGCAGCCAATGCATTGGAACTGCAGAACCAATGCATTGGATGCAT02525050>90ClaICATCGATGGATCGATCCCATCGATGGCCCATCGATGGG00>905000>9050EcoRIGGAATTCCCGGAATTCCGC

3、CGGAATTCCGG>90>90>90>90>90>90精选word范本！.HaeIIIGGGGCCCCAGCGGCCGCTTTGCGGCCGCAA>90>90>90>90>90>90HindIIICAAGCTTGCCAAGCTTGGCCCAAGCTTGGG00100075KpnIGGGTACCCGGGGTACCCCCGGGGTACCCCG0>90>900>90>90MluIGACGCGTCCGACGCGTCG025050NcoICCCATGGGCATGCCATGGCATG050075NdeICCATATGGCCCATATGGGCGCCATATGGCG

4、GGGTTTCATATGAAACCCGGAATTCCATATGGAATTCCGGGAATTCCATATGGAATTCCC000075750000>90>90NheIGGCTAGCCCGGCTAGCCGCTAGCTAGCTAG0101002550NotITTGCGGCCGCAAATTTGCGGCCGCTTTAAAATATGCGGCCGCTATAAAATAAGAATGCGGCCGCTAAACTATAAGGAAAAAAGCGGCCGCAAAAGGAAAA0101025250101090>90NsiITGCATGCATGCACCAATGCATTGGTTCTGCA

5、GTT10>90>90>90PacITTAATTAAGTTAATTAACCCTTAATTAAGG000025>90PmeIGTTTAAACGGTTTAAACCGGGTTTAAACCCAGCTTTGTTTAAACGGCGCGCCGG0007502550>90精选word范本！.PstIGCTGCAGCTGCACTGCAGTGCAAACTGCAGAACCAATGCATTGGAAAACTGCAGCCAATGCATTGGAACTGCAGAACCAATGCATTGGATGCAT010>90>900010>90>900PvuICCGATCGGATCGATCGATTC

6、GCGATCGCGA010002510SacICGAGCTCG1010SacIIGCCGCGGCTCCCCGCGGGGA0500>90SalIGTCGACGTCAAAAGGCCATAGCGGCCGCGCGTCGACGTCTTGGCCATAGCGGCCGCGGACGCGTCGACGTCGGCCATAGCGGCCGCGGAA0101005075ScaIGAGTACTCAAAAGTACTTTT10752575SmaICCCGGGCCCCGGGGCCCCCGGGGGTCCCCCGGGGGA0010>90101050>90SpeIGACTAGTCGGACTAGTC

7、CCGGACTAGTCCGCTAGACTAGTCTAG101000>90>905050SphIGGCATGCCCATGCATGCATGACATGCATGCATGT001002550StuIAAGGCCTTGAAGGCCTTCAAAAGGCCTTTT>90>90>90>90>90>90XbaICTCTAGAGGCTCTAGAGCTGCTCTAGAGCACTAGTCTAGACTAG0>9075750>90>90>90XhoICCTCGAGGCCCTCGAGGGCCGCTCGAGCGG0101002575精选word范本！.XmaICCCCGGGGCCCCCG

8、GGGGCCCCCCGGGGGGTCCCCCCGGGGGGA02550>9007