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1、平颤颗粒治疗帕金森病药效学探究【摘要】目的：观察平颤颗粒对帕金森病(PD)模型鼠的行为及多巴胺能神经元的影响并探讨其治疗PD的作用机制.方法：运用6軽基多巴胺两点注射法损毁大鼠右侧黑质致密部(SNC)和腹侧被盖区(VTA)多巴胺能神经元，制备PD模型，将模型成功大鼠随机分为模型组、美多芭组、平颤颗粒低、中、高剂量3组以及联合用药组，另设正常对照组,每组10只，分别给予相应处理，连续5wk观察各组大鼠处理前及处理后3,4,5wk行为学的变化，通过脑黑质酪氨酸務化酶(TH)免疫组化染色观察多巴胺(DA)
2、能神经元的损伤程度，并采用高效液相色谱法观察脑黑质DA及3,4二軽基苯乙酸(DOPAC)含量变化.结果：平颤颗粒高、中剂量组大鼠的旋转次数多于美多芭组及联合用药组(P<；0.05),但均少于模型组(P<；0.05或P<；0.01),模型组大鼠与治疗前比较旋转次数有显著增加(P<；0.05),正常组大鼠行为则无变化；免疫组化检测显示平颤颗粒高、中剂量组黑质TH阳性神经元数目髙于模型组，但低于正常组(P<；0.05)；高效液相色谱电化学检测结果表明，各组大鼠中脑DA及D0PAC含量均
3、低于正常组,平颤颗粒高、中剂量组低于联合用药组及美多芭组(P<；O.05),而平颤颗粒低剂量组与模型组比较差异无显著性.结论：平颤颗粒能部分改善PD大鼠旋转行为，并减轻黑质细胞的受损程度，提高下降的DA水平，从而对PD起到治疗作用.【关键词】帕金森病；平颤颗粒；旋转行为；酪氨酸单氧化酶；多巴胺[Abstract]AIM：ToobservetheeffectsofPingchanPotionontherotationactionandthedopaminergicneuroninParkinson
4、disease(PD)modelratsandexplorethepharmacodynamicsofPingchanPotion.METHODS：PDmodelratswereestablishedbymicroinjectionof6hydroxydopaminesolutionintorightventraltegmentalarea(VTA)andsubstantianigraparscompacta(SNC)ofthemesencephslon.The60PDratsweredivided
5、randomlyintosixgroups：modelgroup,madopargroup,PingchanPotion(16,8,4g/kg)groupandcombinedgroup.Inaddition,tenratswereineludedinthenormalcontrolgroup.Allratswerecontinuallytreatedfor5weeks.Therotationalbehaviorofratsbeforeandaftertreatmentbyapomorphinewa
6、sobserved.After5weeks,thenumberoftyrosinehydroxylase(TH)positivecellsweredetectedbyimmunohistochemicalmethod,anddopamine(DA)anditsmetabolicproductsinnigrostriatalsystemofeachgroupweredeterminedbyusinghighperformaneeliquidchromatographycoupledtoelectroc
7、hemicaldetection(HPLCECD).RESULTS：Comparedwithmodelgroup,thetimesofratsrotationweredecreasedrespectivelyafter3weektreatmentinthemadopargroupandcombinedgroup(P&It；0.05)；thetimesofratsrotationdidntdecreaseinPingchanPotion16g/kgand8g/kggroup,butitdidnotch
8、angeobviouslyin4g/kggroup(P>；0.05)；thetimesofratsrotationinmodelgroupincreasedposttreatmeat(P&It；0.05),andthebehaviourofnormalgroupdidntchange.ThenumberofTHpositivecellsinsubstantialnigraofra/tstreatedwithPingchanPotion16g/kgand8g/kgw
