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1、三分群血细胞研究仪血小板计数结果影响因素研究［摘要］目的对影响三分群血细胞分析仪血小板检测结果的相关因素进行分析，寻求解决假性异常结果的途径和方法，避免临床误诊。方法随机选取门诊和住院患者100例,其中，末梢血50例，静脉血50例，均采用乙二胺四乙酸二钾(EDTA—K2)进行抗凝后30min内上机检测，异常结果行手工计数和血涂片染色观察。结果100例原始标本中共有异常结果11例，包括减低8例，增高3例；通过更换抗凝剂、手工计数、涂片染色观察，6例减低结果被纠正，2例为EDTA抗凝剂依赖引起的假性血小板减少症(EDTA—PTCP),3
2、例属反应性增高。结论当仪器出现与临床诊断严重不符的血小板异常结果时，应及时分析原因，通过更换抗凝剂种类、适当控制标本放置时间，同时辅以手工计数和血涂片染色观察可有效区分真性与假性计数结果异常，提高检测结果的准确性。［关键词］血细胞分析仪；血小板计数；抗凝剂；影响因素［中图分类号］R446.11+1［文献标识码］A［文章编号］1674—4721(2012)09(c)—0086—02Analysisofinfluencefactorsofplateletcountresultsofthree一clusterbloodcellanalyz
3、erLIUJuDepartmentofLaboratory,MaternalandChildCareServiceCenterofYunCountyinShiyanCityinHubeiProvince,YunCounty442500,China[Abstract]ObjectiveToanalyzetherelatedinfluencefactorsofplateletcountresuItsofthree一clusterbloodcellanalyzer,andseekwaysandmeansofsolvingpseudoexc
4、eptionresults,soastoavoidclinicalmisdiagnosis.MethodsOnehundredcasesofout一patientsandin一patientswerechosenrandomly,with50casesofperipheralbloodand50casesofvenousblood.Allpatientsweremadethetestbythemachinewithin30minutesafteranticoagulantbyethylenediaminetetraaceticaci
5、ddipotassium（EDTA一K2）.Theabnormalresultswerecountedbyhandandobservedbybloodsmearstaining.ResuItsIntheoriginalsamplesof100cases,therewere11casesofabnormalresults,including8casesofdecreasedand3casesofincreased・Throughtheexchangeofanticoagulant,manualcountandobservationof
6、smearstaining,6casesofdecreasedwerecorrected,2caseswerepseudo一thrombocytopenia(EDTA一PTCP)causedbyEDTAanticoagulantresistance，3caseswereincreasedreactivity・ConclusionWhentheequipmentsshowthatabnormalplateletresuItsdon'tcomplywithclinicaldiagnosisseriously,itisnecessaryt
7、oanalyzethereasonsintime・Throughexchangingthetypesofanticoagulant，controllingstoragetimeofsamplesappropriatelyandsuppplementedbymanualcountandobservationofbloodsmearstaining,thetrueandfalsecountabnormalresultscanbedistinguishedeffectively,andtheaccuracyoftestresuItscan
8、beimproved・[Keywords]Bloodcellanalyzer；Plateletcount；Anticoagulant；Influencefactor三分群血细胞分析仪以其准确、快速、经济适用广泛应用于各级医院，由于其采
