资源描述：
《人骨髓间质干细胞对肝星状细胞的体外调控》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、中国组织工程研究与临床康复第13卷第27期2009–07–02出版JournalofClinicalRehabilitativeTissueEngineeringResearchJuly2,2009Vol.13,No.27基础医学人骨髓间质干细胞对肝星状细胞的体外调控****☆1111121胡昆鹏，林楠，林继宗，邓美海，汤照峰，项鹏，许瑞云Invitroregulationeffectofhumanbonemarrowmesenchymalstemcellsonhepaticstellatecells1111121HuKun-pe
2、ng,LinNan,LinJi-zong,DengMei-hai,TangZhao-feng,XiangPeng,XuRui-yunAbstract1BACKGROUND:Thereisnoacceptedtreatmentforliverfibrosisrecently.Bonemarrowmesenchymalstemcells(BMSCs)usedDepartmentofinthetreatmentofliverfibrosishasbeenreportedasaneffectivelytreatment,butthemec
3、hanismisunclear.HepatobiliarySurgery,ThirdAffiliatedHospital,OBJECTIVE:TostudytheregulationofhepaticstellatecellsmediatedbyhumanBMSCsinvitro.SunYat-senUniversity,DESIGN,TIMEANDSETTING:ThecytologicalinvitrostudywasperformedattheCenterforStemCellsandTissueEngineeringGua
4、ngzhou510630,ofSunYat-senUniversityandtheCentralLaboratoryofThirdAffiliatedHospitalofSunYat-senUniversityfromJunetoGuangdongProvince,2December2008.China;ResearchCenterofStemcellsMATERIALS:Humanbonemarrowmesenchymalstemcellswerecollectedfromnormalyouthvolunteers;Humanh
5、epaticandTissuestellatecellsandnormallivercelllineL-O2weresuppliedbytheAnimalExperimentalCenterofSunYat-senUniversity.Engineering,SunMETHODS:ThepurifiedhumanBMSCsandhepaticstellatecellsweresetupinTranswellco-culturesystem.TheincubationYat-senUniversity,densitywas2×104
6、cells/well.L-O2wassetupinsteadofhumanBMSCsasnegativecontrol.HepaticstellatecellsculturedaloneGuangzhou510080,GuangdongProvince,servedasblankcontrolgroup.Theculturewasperformedfor72hours.ChinaMAINOUTCOMEMEASURES:Morphologyofhepaticstellatecellsandresultsofimmunocytoche
7、micalstaining.Apoptosisofhepaticstellatecellswasdeterminedbyflowcytometry.Westernblotwereusedtoassaytheexpressionofα-actin.HuKun-peng☆,RESULTS:Activatedhepaticstellatecellspresentedflatandthinshapeunderaninvertedmicroscope.FatdropwaslackinStudyingfordoctorate,Physicia
8、n,Departmentcytoplasm.α-actinlocatedinhepaticstellatecells,withthepresenceofhightensionfibers.ComparedwiththeL-O2+hepaticofH
