结直肠癌中抑癌基因ING1表达探究

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1、结直肠癌中抑癌基因ING1表达探究作者：张森韦建宝周永淳陈利生【摘要】目的探讨抑癌基因ING1及其蛋白p33/INGl在结直肠癌中的表达。方法用RTPCR检测35例散发性结直肠癌中ING1mRNA的表达水平，并应用SP法检测60例散发性结直肠癌及正常黏膜组织中P33/ING1蛋白的表达。结果结直肠癌组织、正常黏膜组织中P33/ING1蛋白阳性表达率分别为43.3%(26/60)、100%(60/60),两者比较差异有统计学意义(P<；0.01)op33/INGl在无淋巴结转移组及淋巴结转移组中的阳性

2、表达率分别为57.6%(19/33)、25.9%(7/27),两者比较差异有统计学意义(P<；0.05)o在DukesA.B期和DukesC、D期患者癌组织中p33/ING1的阳性表达率分别为56.7%(17/30)、30.0%(9/30),两者比较差异有统计学意义(P<；0.05)o结论ING1及p33/ING1蛋白的低表达与散发性结直肠癌发生、发展密切相关，临床分期越差者表达水平越低。【关键词】抑癌基因ING1结肠肿瘤直肠肿瘤[Abstract]ObjectiveTodiscusstheex

3、pressionoftumorsuppressorgeneING1anditsproteinp33/INGlindevelopmentofsporadiccolorectaicarcinoma.MethodsReversetranscriptionpolymerasechainreactionwasusedtodetectmRNAexpressionlevelofING1in35caseswithsporadiccolorectalcarcinomaandstreptomyceteavidinperox

4、idaseimmunohistochemistrytomeasureexpressionlevelofproteinp33/INGlincolorectaicanceroustissuesandnormalmucoustissuesfrom60caseswithsporadiccolorectaicarcinoma.ResuItsExpressionlevelofp33/INGlmRNAincolorectaicanceroustissueswasmoresignificantlydecreasedth

5、anthatinnormalmucoustissues・Expressionlevelofp33/INGlmRNAinthetumorsofpatientsatstageofDukesAandBwassignificantlyhigherthanthatinpatientsatstageofDukesCandD,asindicatedthattheexpressiondecreasedwithpoorclinicalstage.Positiveexpressionrotesofp33/INGlincol

6、orectaicanceroustissuesandnormalmucoustissueswere43.3%(26/60)and100%(60/60),respectively,withsignificantstatisticaldifference(P&It；0.01)・Positiveexpressionratesofp33/INGlingroupswithorwithoutlymphnodemetastasiswere25.9%(7/27)and57.6%(19/33)respectively(P

7、<；0.05).Positiveexpressionratesofp33/INGlinpatientsatstageofDukesA,BandatstageofDukesCandDwere56.7%(17/30)and30.0%(9/30)respectively(P<；0.05).ConclusionsThedecreasedexpressionofp33/INGlandlowexpressionofproteinp33/INGlarecloselyrelatedwithdevelopment

8、ofsporadiccolorectaicarcinoma・[Keywords]Tumorsuppressorgene；Inhibitorofgrowth；Coloncancer；Rectaicancer结直肠癌是临床上一种常见的恶性肿瘤。随着分子生物学研究的进展，结直肠癌的发生被认为与癌基因的功能增强、抑癌基因功能低下以及DNA错配修复基因缺失等因素密切相关。ING1(inhibitorofgrowth)基因是近年来克隆出来的一个抑癌基因