EPA Method 8318 N-Methylcarbamates by High Performance Liquid Chromatography

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1、METHOD8318N-METHYLCARBAMATESBYHIGHPERFORMANCELIQUIDCHROMATOGRAPHY(HPLC)1.0SCOPEANDAPPLICATION1.1Method8318isusedtodeterminetheconcentrationofN-methylcarbamatesinsoil,waterandwastematrices.Thefollowingcompoundscanbedeterminedbythismethod:_________________

2、______________________________________________________________aCompoundNameCASNo.________________________________________________________________________________Aldicarb(Temik)116-06-3AldicarbSulfone1646-88-4Carbaryl(Sevin)63-25-2Carbofuran(Furadan)1563-

3、66-2Dioxacarb6988-21-23-Hydroxycarbofuran16655-82-6Methiocarb(Mesurol)2032-65-7Methomyl(Lannate)16752-77-5Promecarb2631-37-0Propoxur(Baygon)114-26-1________________________________________________________________________________aChemicalAbstractServicesR

4、egistryNumber.1.2Themethoddetectionlimits(MDLs)ofMethod8318fordeterminingthetargetanalytesinorganic-freereagentwaterandinsoilarelistedinTable1.1.3Thismethodisrestrictedtouseby,orunderthesupervisionof,analystsexperiencedintheuseofhighperformanceliquidchro

5、matography(HPLC)andskilledintheinterpretationofchromatograms.Eachanalystmustdemonstratetheabilitytogenerateacceptableresultswiththismethod.2.0SUMMARYOFMETHOD2.1N-methylcarbamatesareextractedfromaqueoussampleswithmethylenechloride,andfromsoils,oilysolidwa

6、steandoilswithacetonitrile.Theextractsolventisexchangedtomethanol/ethyleneglycol,andthentheextractiscleaneduponaC-18cartridge,filtered,andelutedonaC-18analyticalcolumn.Afterseparation,thetargetanalytesarehydrolyzedandderivatizedpost-column,thenquantitate

7、dfluorometrically.2.2Duetothespecificnatureofthisanalysis,confirmationbyasecondarymethodisnotessential.However,fluorescenceduetopost-columnderivatizationmaybeconfirmedbysubstitutingtheNaOHando-phthalaldehydesolutionswithorganic-freereagentwaterandreanaly

8、zingthesample.IfCD-ROM8318-1Revision0September1994fluorescenceisstilldetected,thenapositiveinterferenceispresentandcareshouldbetakenintheinterpretationoftheresults.2.3Thesensitivityofthemethodusuallydependsonthelevelofinte