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1、卵巢癌中MDR1的表达及其临床意义作者：梁川，吴绪峰,陈慧君单位：武汉大学中南医院妇瘤科【摘要】目的检测MDR1在卵巢癌中的表达并探讨其在卵巢癌化疗耐药中的作用。方法采用RTPCR复合定量技术対40例卵巢癌、20例良性卵巢肿瘤、20例止常卵巢组织中MDR1的表达进行检测，分析其表达与临床病理特征及化疗疗效的相关性。结果卵巢癌中MDR1的表达显著高于正常组及良性肿瘤组，且肿瘤分化程度越差其表达越高，P<0.05。MDR1在术前化疗组屮的阳性表达率就著高于术前未化疗组，P<0.05oMDRI阴性组患者的化疗反应率显著高于阳性组患者(70.
2、6%比43.5%),P<0.05,MDR1阴性对化疗有效的预测值为70.6%o结论MDRInJ能在卵巢癌获得性耐药的形成中发挥一定作用，MDR1的检测可能有助于卵巢癌化疗方案的制定，并成为预测化疗疗效的有用指标。【关键词】卵巢癌；多药耐药基因；逆转录聚合酶链反应ExpressionofMultidrugResistanceGeneinEpithelialOvarianCarcinomaandItsClinicalSignificanceLIANGChuan,WUXufeng,CHENHuijunGynecologicOncologyDepartm
3、entofZhongnanHospital,WuhanUniversity,Wuhan430071,ChinaCorrespondingAuthor:WuXufeng,Email：wuxufeng@371.netAbstract：ObjectiveTodetecttheexpressionofmultidrugresistancegene(MDRI)intheepithelialovariancarcinoma,andinvestigateitsroleindrugresistance.MethodsTheexpressionofMDRIin40
4、casesofovariancarcinoma,20casesofbenignovariantumorand20casesofnormalcontrolweredeterminedbyRTPCRtechnique.TherelationsbetweenMDRIexpressionandtheclinicalpathologicalcharacteristicsaswellaschemotherapeuticresponsewerediscussecl.ResultsThepositiveexpressionrateofMDRIinovarianc
5、arcinomawassignificantlyhigherthanthatinbenigntumorsandnormaltissues,andasignificantrelationshipwasshownbetweenitsexpressionandpathologicdifferentiation,P<0.05・TheMDRIpositiveexpressionrateincasesreceivedchemotherapybeforesurgeryweresignificantlyhigherthanincaseswhodidn't,
6、P<0.05・TheresponserateintheMDRInegativegroupwashigherthanthatinthepositivegroup(70.6%vs43.5%),P<0.05.ThepredictivevalueofnegativeMDRIstainingforobjectiveresponsewas70.6%.ConclusionThesedatasuggestedthatMDRImayplayanimportantroleintheacquiredmultidrugresistanceofovarianc
7、arcinoma.DeteiminationofMDRIexpressionmaybeusefulforworkingoutchemotherapyplanandpredictingchemotherapeuticresponseofovariancarcinomapatients.Keywords：Ovariancarcinoma;Multidrugresistancegene;Reversetranscriptionpolymerasechainreaction虽然卵巢癌对化疗相对敏感，但其远期效果并不理想，据报道尽管卵巢癌首次化疗有效率高达
8、76%,但复发后降至20%[1],药物耐受是化疗失败的重要原因。肿瘤耐药的产生是多棊因参与的复杂过程，其中以多药耐药基因MDRI(mul
