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1、16.15.06(h)Calibratedflowmeters.—Six,range(1.0–5.0L/min),one,AOACOfficialMethod997.17(5.0–30.0L/min)(DwyerInstruments,POBox373,MichiganCity,MicrobialRankingIN46361,USA,VFA-23BV,orequivalent).ofPorousPackagingMaterials(i)Sterilecellulosenitratefilters.—Forcolle
2、ctionofspores,47(ExposureChamberMethod)or50mmdiameter,0.45mmporosity(SartoriusCorp.,131Heart-ASTMMethod(1)landBlvd,Edgewood,NY11717,USA).FirstAction1997(j)Bottle-topfilterunits.—Toprovidethemainhousingofat-SeeTable997.17Afortheresultsoftheinterlaboratorystudys
3、up-tachmenttothechamberwhichcontainthematerialtestedandtheportingtheacceptanceofthemethod.membranefilterforsporecollection(FalconType7104,[BectonDickinsonLabware,1BectonDr,FranklinLakes,NJ07417-1880,A.PrincipleUSA,Cat.No.357104],orNalgenefilterholderswithfunne
4、lTestportionsofporousmaterialsaresubjectedtoanaerosolof310–4000,orequivalent).Bacillussubtilisvarnigerspores(ATCC9372)withinanexposure(k)Glassnebulizer.—Foratomizationofsporesintothechamber.Sporeswhichpassthroughtheporousmaterialarecol-chamber(DeVilbiss,Sunris
5、eMedical,100DeVilbissDr,lectedonmembranefiltersandenumerated.Thelogarithmreduc-Somerset,PA15501,USA,No.45orequivalent).tionvalue(LRV)iscalculatedbysubtractingthelogarithmofthe(l)Sterileforceps.—Forremovalofmembranesfromthebottle-numberofsporespassingthroughthe
6、porousmaterial(N1)fromthetopfilterunits,5in.(12.7cm)orlonger.logarithmoftheinitialmicrobialchallenge(N0).(m)Disccutter.—Topreparematerialsfortesting,47or50mmB.Apparatusdiameter(DiecraftCo.,807ParkAve,Minneapolis,MN55404-1136,USA,orequivalent).(a)Exposurechambe
7、r.—Thechamberisconstructedofacrylic(n)Sterilesyringe.—Toinjectthesporesuspensionintothesheetingandconsistsof2sectionsasillustratedinFigure997.17A.nebulizer(3cm3,20gauge,11in.[3.81cm]longneedle)orThebottomsectioncontainsa6-placemanifoldconnectedto26flowmeters,o
8、neperport,containinghosesattachedto6filteringmicropipette.units.Theporttothemanifoldisattachedtoavacuumsource.Avac-(o)Sterilepipets.—Todeliver0.1,1,10,and25mLaliquots.uumgaugeismou
