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1、41.1.66detectedbydifferentialrefractometry.Quantitationisbyareanor-AOACOfficialMethod993.24malization.Triglycerides(byPartitionNumbers)ElutionorderisdeterminedbycalculatingequivalentcarboninVegetableOilsnumbers,ECN,oftendefinedasCN–2n,whereCNiscarbonnum-LiquidChromatographicMethodberand
2、nisnumberofdoublebonds.TocalculateECNmorepre-FirstAction1993cisely,originofthedoublebondistakenintoaccount.FinalAction1996ECN=CN–dono–dlnl–dlnnlnIUPAC–AOCS–AOACMethodwhereno,nl,andnlnarenumberofdoublebondsofoleic,linoleic,(Applicabletodeterminationoftriglyceridesoflong-chainfattyac-andl
3、inolenicacids,respectively,andcoefficientsdo,dl,anddlnareidsinvegetableoils.)calculatedfromreferencetriglycerides.Underconditionsinthismethod,ECNapproximates:SeeTable993.24fortheresultsoftheinterlaboratorystudysup-portingacceptanceofthemethod.ECN=CN–(2.60´no)–(2.35´nl)–(2.17´nln)A.Princ
4、ipleB.ApparatusTriglyceridesinvegetableoilsareseparatedaccordingtoequiva-(a)Liquidchromatographic(LC)system.—Equippedwithther-lentcarbonnumberbyreversed-phaseliquidchromatographyandmostaticcontrolofcolumntemperature;10mLinjectionunit;differ-Table993.24Interlaboratorystudyresultsfordeter
5、minationoftriglyceridesinvegetableoils,basedonresultsfrom2studies,16and18laboratoriesTestsampleECN-40ECN-42ECN-44ECN-46ECN-48ECN-50SoybeanoilMean,%8.225.531.021.610.71.5RSDr3.41.81.33.57.314.5RSDR6.73.53.94.39.571.7r0.81.31.12.12.20.6R1.62.63.42.72.93.1AlmondoilMean,%0.12.213.030.750.44
6、.4RSDr—18.84.41.71.65.4RSDR—27.86.92.32.79.1r—1.21.61.52.30.7R—1.82.62.03.91.1SunfloweroilMean,%0.329.438.422.58.21.3RSDr21.41.81.21.610.737.0RSDR70.43.23.42.411.792.6r0.51.51.31.02.51.4R0.62.73.71.62.73.4OliveoilMean,%—0.65.921.264.77.2RSDr—16.21.51.30.83.6RSDR—21.13.01.61.68.5r—0.30.3
7、0.81.50.7R—0.30.51.02.91.7RapeseedoilMean,%3.19.822.928.731.23.0RSDr5.31.80.71.71.713.2RSDR8.52.32.82.42.540.8r0.50.50.51.41.51.1R0.70.61.82.02.23.4PalmoilMean,%——3.322.164.79.4RSDr——8.12.31.29.6RSDR——16.22.82.39.6r——0.81.42.32.5R——1.51.84.22.5©2000AOACINTERNATIONALFigure993.24
