资源描述：
《AOAC 32.2.02A AOAC Official Method 996.01 Fat》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、32.2.02A(c)Monjonnierfatextractionflask.—25mL,KontesGlass,1022AOACOfficialMethod996.01SpruceSt,POBox1512,Vineland,NJ08362USA,PartnumberFat(Total,Saturated,Unsaturated,and617600-0025.Monounsaturated)(d)Waterbaths.—Maintaining(1)80±2°C;and(2)maintaininginCerealProductsambienttemperature.AcidHydrol
2、ysisCapillaryGasChromatographicMethod(e)Oven.—Maintaining103±2°C.FirstAction1996(f)Boilingchips.—Fatfree.(g)Cotton.—Defatted;alternatively,glasswoolcanbeused.(Applicablefordeterminationoffatincerealproductscontaining(h)Glassfunnel.—60°angle,fluted.0.5–13%totalfat.)(i)Steamtable.(j)Testtubes.—22´
3、275mm,Pyrex,screwtop,withcap.SeeTables996.01A–Dfortheresultsoftheinterlaboratorystudy(k)Testtuberack.supportingtheacceptanceofthemethod.(l)Bottle.—Fordispensingacid,1Lwith15mLdispensingbulb,orequivalent.A.Principle(m)Vortexmixer.Totallipidextractisobtainedbydigestingtestportionwithhot(n)Reaction
4、flasks.—250mL,flat-bottomboilingflaskswithHCl.Hydrolyzedfatcomponentsareextractedintoethylandpetro-24/40joint.leumethers,andthenevaporated.Theextractissaponifiedand(o)Condenser.—Water-cooledrefluxcondenser,with40–50cmmethylated.Fattyacidmethylestersaredeterminedbycapillarygasjacketand24/40joint.
5、chromatography.Totalfatiscalculatedassumofindividualfattyac-(p)Centrifugetubes.—50mL,conical.idsexpressedastriglycerideequivalents.Saturated,unsaturated,(q)Centrifuge.—Maintaining1875´g.andmonounsaturatedfatsarecalculatedassumofindividualfatty(r)Grindingmill.—With0.5mmscreen.acids.Methodcanbeuse
6、dforquantitationoftransisomerswhenus-ingappropriatestandardsoffattyacidmethylesters.C.ReagentsB.Apparatus(a)Ethanol.(a)Gaschromatograph.—Withcapillarysplit/splitlessinjection(b)Methanol.systemandflameionizationdetector.Operatingconditions:tempera-(c)Hydrochloricacid.—8M(25+11;v/v).tures;injector
7、,250°C;detector,275°C;Hflow,34mL/min;airflow,(d)Ethylether.—Peroxide-free.ca300mL/min;splitratio,100:1;carriergas,He;linearvelocity,(e)Petroleumether.—Peroxide-free.21cm/sat175°C;initialtemp,120°C(hold4min);rate,5.0°C/min;(f
