资源描述：
《AOAC 4.2.11 AOAC Official Method 2001.11 Protein(Crude)》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、4.2.11tionproceduresmustbeusedinordertoincludeNfromnitrate.)TheAOACOfficialMethod2001.11amountofproteininmostmaterialsiscalculatedbymultiplying%NProtein(Crude)inAnimalFeed,by6.25,becausemostproteinscontain16%N.Forage(PlantTissue),Grain,andOilseedsTheH2SO4andN
2、aOHusedareinconcentratedformandareBlockDigestionMethodUsingCopperCatalysthighlycorrosive.WearglovesandeyeprotectionwhilehandlingtheandSteamDistillationintoBoricAcidchemicals.DonotmixconcentratedacidandNaOHdirectly.IfFirstAction2001chemicalsaresplashedontheski
3、norintheeyes,flushwithcopious[Applicabletothedeterminationof0.5–50%KjeldahlNamountsofwater.Seekmedicalattention.Donotbreathethesulfur(3–300%equivalentcrudeprotein)inforage,animalfeedandpetoxidefumesproducedduringdigestion.food,grain,andoilseeds,andapplicablet
4、othesamematrixesasB.Apparatus976.05(4.2.05),976.06(4.2.06),984.13(4.2.09),988.05(4.2.03),(a)Digestionblock.—Aluminumalloyblockwithadjustableand990.02(4.2.07);themethoddoesnotmeasureoxidizedformsoftemperaturedeviceformeasuringandcontrollingblocktemperatureNorh
5、eterocyclicNcompounds.](TecatorDigestionSystem20,1015Digestor,FossNorthAmerica,SeeTables2001.11AandBfortheresultsoftheinterlaboratory7682ExecutiveDr,EdenPrairie,MN55344,USA;study,expressedonaproteinbasis(N´6.25),supportingaccep-+1-952-974-9892,Fax:+1-952-974-
6、9823,tanceofthemethod.info@fossnorthamerica.com;orequivalent).(b)Digestiontubes.—250mL.A.Principle(c)Distillationunits.—(1)Forsteamdistillation.—FossThematerialisdigestedinH2SO4toconverttheproteinNtoTecator2200,orequivalent,toaccept250mLdigestiontubesand(NH4)
7、2SO4ataboilingpointelevatedbytheadditionofK2SO4with500mLtitrationflasks.(2)ForsteamdistillationandaCucatalysttoenhancethereactionrate.Ammoniaisliberatedbyautotitration.—FossTecator2300,orequivalent.alkalinesteamdistillationandquantifiedtitrimetricallywithstan
8、-(d)Titrationflask.—500mLgraduatedErlenmeyerflask(fordardizedacid.Aluminumblockheatersincreasetheefficiencyofthecollectionandtitrationofdistillate).digestion.(e)Fumeexhaustmanifold.—WithT
