不同抗虫棉品种杀虫蛋白表达分析及影响抗虫性分析

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1、influencewasdifferentwithgenerationnumberunderdifferentgeneticbackground・3)Comparedtwosetofpedigreematerials,thecontentofBTproteininL2000-2andL169originedNorthwestinlandregionweresignificantlyhigherthanthatofLufamilycottonoriginedYellowRiverBasin.So,itindicatedthatt

2、hegeneticbackgroundwasonefactortoinfluenceBTcontent.Inaddition,weanalyzedmanymaterialswithdifferentbackgroundandtheresultshowedthatenvironmentwasanotherfactortoinfluenceBTcontentandtheeffectsofenvironmentwasbeaffectedbygeneticbackground-4)Theexperimentofmixvarieties

3、showedthatcottonbollwormdamagelevelwasgraduallyincreased,themortalityrateofH.armigerawasdecreasedandtheyieldwassignificantlyreducedwithseedpuritydecreased・Andthefactorsofyieldreductionwereresultfromdecliningininsectresistance,thenumberoffruitbranchesandbollnumber.In

4、conclusion,thedeclineofseedpurityhadsignificanteffectiononinsectresistanceandcottonyeild・5)Bymodifiedsafetyassessmenttestingandmonitoring,theBTcontentinimprovedvarietieswasn'tsignificantdifferencewiththatoflocalmainvarieties・Itshowedthattheimprovedvarietieshadbetter

5、resistanceoncottonbollwormbecausetheaveragecorrectedmortalityofcottonbollwonnwasabove80%andthehazardlevelwaslowerthan2.Insummary,theexpressionofBTinsecticidalproteinintransgenicBtcottonhadbeencomprehensivelyanalyzedusing30transgenicBtcottonvarietiesinChinaand1transg

6、eniccottoninAmerican・ThesecommercialvarietieshadhighexpressionlevelofBTproteinandhadbetterresistanceoncottonbollworm.So,weconsideredthatthephenomenonofMInsect-resistantcottonisnotncouldonlybecausedbymixedspecies.Fromdifferentgenerationandgenealogymaterials,wefurther

7、provedthattheenvironmentalandgeneticbackgroundhadinfluenceonexpressionofinsecticidalprotein・Thisstudyprovidedatheoreticalbasisaboutseedselectionofhighinsectresistantcottonvarietiesforbreeders・Theresultsofsafetyassessmentshowedthattheenvironmentalreleaseoftransgenic

8、cottonwassafety・Butthefurtherstudywillbedone・Keywords：transgeniccotton;insecticidalproteinexpression;environment;geneticbackground;safetya