SCF在新生大鼠卵巢镉暴露致卵母细胞凋亡及其相关基因表达中的作用

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1、分类号：R114学校代码：10392学科专业代码：100405学号：2110705630福建医科大学硕士研究生毕业论文SCF在新生大鼠卵巢镉暴露致卵母细胞凋亡及其相关基因表达中的作用TheeffectofSCFonoocytesapoptosisandrelatedgeneexpressioninducedbycadmiumexposureinnewbornratovaries学位类型：医学硕士学位所在学院：公共卫生学院研究生：谢美美学科、专业：卫生毒理学导师：张文昌教授研究起止日期：2011年9月至2014年6月答辩日期：2014年6月3日报告日期：2014年6月万方数据福建医科大学硕士

2、研究生毕业论文1万方数据福建医科大学硕士研究生毕业论文目录学位论文原创性声明和版权使用授权书„„„„„„„„„„„1英文缩写词汇表„„„„„„„„„„„„„„„„„„„„„3中文摘要„„„„„„„„„„„„„„„„„„„„„„„„5ABSTRCT„„„„„„„„„„„„„„„„„„„„„„„„„9前言„„„„„„„„„„„„„„„„„„„„„„„„„12技术路线图„„„„„„„„„„„„„„„„„„„„„„„13SCF在新生大鼠卵巢镉暴露致卵母细胞凋亡及其相关基因表达的中的作用„„„„„„„„„„„„„„„„„„„„„„„„„„17引言„„„„„„„„„„„„„„„„„„„„„„„„17材

3、料与方法„„„„„„„„„„„„„„„„„„„„„17实验结果„„„„„„„„„„„„„„„„„„„„„„31讨论„„„„„„„„„„„„„„„„„„„„„„„„58全文结论„„„„„„„„„„„„„„„„„„„„„„„„68参考文献„„„„„„„„„„„„„„„„„„„„„„„„69致谢„„„„„„„„„„„„„„„„„„„„„„„„„„83综述„„„„„„„„„„„„„„„„„„„„„„„„„„84附录„„„„„„„„„„„„„„„„„„„„„„„„„„922万方数据福建医科大学硕士研究生毕业论文英文缩写词汇表（按英文字母顺序）英文缩写英文全称中文名词β-actinβ-actinβ-肌

4、动蛋白ANOVAanalysisofvariane方差分析BCL-2Bcelllymphoma/lewkmia-2B细胞淋巴瘤/白血病-2BCL-XLB-celllymphoma-extralargeB细胞淋巴瘤BAKBcl-2antagonist/killerBcl-2的拮抗剂/杀手BAXBcl-2-associatedXproteinBcl-2的相关X蛋白CdCadmium镉cDNACompletementaryDNA互补DNAdpcdayspostcoitum交配后DEPCDiethylpyrocarbonate焦碳酸二乙酯FSHfolliclestimulationhormone促

5、卵泡激素GVBDgerminalvesiclebreakdown生发泡破裂HEhematoxylin-eoxin苏木素-伊红IQIntelligenceQuotient智商KLKitLandKit配体LHluteinizinghormone黄体生成素mRNAMessengerRNA信使RNANF-κBnuclearfactorkappaB核因子κBODopticaldensity光密度值PBSPhosphatebuffersolution磷酸盐缓冲溶液PCDprogrammedcelldeath程序性细胞死亡PCRPolymeraseChainReaction聚合酶链反应PNDpostna

6、taldays出生后日龄Realtime-PCRRealtime-polymeraseChainreaction实时定量聚合酶链反应SCFstemcellfactor干细胞因子SDS-聚丙烯酰氨凝胶电SDS-PAGESDS-polyacrylamidegelelectrophoresis泳3万方数据福建医科大学硕士研究生毕业论文SDSprague-Dawley斯普拉格-杜勒Tmmeltingtemperature解链温度Terminal-deoxynucleotidyl脱氧核糖核苷酸末端转TUNELTransferasemediatednick移酶介导的缺口末端标endlabeling记法

7、Western-blot免疫蛋白印迹4万方数据福建医科大学硕士研究生毕业论文SCF在新生大鼠卵巢镉暴露致卵母细胞凋亡及其相关基因表达中的作用中文摘要目的：观察新生大鼠卵巢镉暴露后卵母细胞的凋亡及其相关基因表达的变化，研究新生大鼠镉暴露致卵母细胞凋亡及其可能的分子机制,进一步探讨SCF在此过程中的作用及其信号通路研究。方法：4日龄雌性SD大鼠，卵巢体外培养，37℃，5%CO2，培养4天。1.染镉剂量分别为0µM、0.5µM