资源描述：
《贺晓杰生化论文》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、齐齐哈尔大学毕业设计（论文）题n低剂量微波辐射对重金属污染下玉米幼苗生长发育的影响学院生命科学与农林学院专业班级生物技术083班学生姓名贺晓杰指导教师吴旭红教授成绩2012年6月20口摘要对浸泡10h后的种子分别釆取Os,5s,10s,15s,20s的低火微波处理(微波强度为180mW/cm2)o用盆栽的方法培养玉米幼苗，7d后测定发芽率，lid后，测发芽指数和活力指数。待玉米长至三叶期后，每天早晚用注射器滴灌5mLCdCl?溶液，溶液设低、中、高3个浓度梯度,即0.01,0.1,1.0mmol/L
2、,对照组(CK)浇等量自来水,3d后釆集玉米叶片测定生理指标。与对照组相比较，低剂量辐射可以提高玉米种子发芽率，高剂量辐射降低发芽率，4种不同时间长度的处理均可以提高蛋白酶的活性，经过高浓度CdCl?处理的玉米幼苗叶片屮的超氧化物歧化酶(SOD)和过氧化物酶(POD)等酶的活性低于对照组，蛋白质的含量低于对照组，叶绿素a、b的含量明显低于对照。这表明，用适宜剂量的微波辐射处理玉米种子能提高玉米的发芽率，有助于玉米幼苗的生长发育，低浓度的镉溶液能够促进玉米幼苗中S0I)和P0I)的活性，高浓度的镉溶液
3、对其产生抑制作用。对叶绿素的影响则表现为低浓度的镉对叶绿素a和叶绿素b均有刺激作用，高浓度的镉对其则起到了抑制作用。关键词：玉米幼苗；微波辐射；氯化镉；发芽指数；蛋口质含量；幼苗发育AbstractSoaktheseedsafterlOh,respectively,totaketheOs,5s,10s,15s,20s,thelow-firemicrowavetreatment(microwaveintensityof180mW/cm2).Potmethodtocultivatemaizeseedli
4、ngs,measuredaftergerminationrate7d,lid,measuredgerminationindexandvigorindex.Cornuptothree-leafstageuntilafterday,morningandeveningdripirrigationwithasyringe5mLofCdChsolution,thesolutionsetlow,mediumandhighconcentrationgradient3,namely0.01,0.1,1.0mmol/
5、L,controlgroup(CK),pouringthesameamounttapwater,collected3daftercornleavesdeterminationofphysiologicalindicators.Comparedwiththecontrolgroup,lowdosesofradiationcanbeimprovedmaizeseedgerminationrate,high-doseradiationtoreducethegerminationrate,fourdiffe
6、rentlengthoftimeprocessingcanimprovetheactivityoftheprotease,superoxideafterthetheCdChprocessingofthehighconcentrationofleavesofmaizeseedlingstheenzymesuperoxidedismutase(SOD)andperoxidase(POD)activitywaslowerthaninthecontrolgroup,theproteincontentislo
7、werthanthecontrolgroup,chlorophyllaandbweresignificantlylowerthanthecontrol.Thissuggeststhatappropriatedosesofmicrowaveradiationprocessingofcomseedlingscanimprovecorngerminationrate,contributetothegrowthanddevelopmentofmaizeseedlings,andlowconcentratio
8、nsofcadmiumsolutioncanpromotetheactivityofSODandPODinmaizeseedlings,highconcentrationsofcadmiumsolutionproducedinhibition.Thechlorophyllperformancelowconcentrationsofcadmiumonchlorophyllaandchlorophyllbhaveastimulatingeffect,highconcent
