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1、雷帕霉素对大鼠脑缺血再灌注损伤的保护效应研究王丽君吴登艳胡安春杨晨景玉宏*1・兰州大学基础医学院（甘肃兰州730000）2•兰州大学基础医学院神经科学研究所（廿肃兰州730000）基金资助：兰州大学本科生创新创业行动计划资助*通讯作者简介：景玉宏，男，I専士，副教授，兰州大学基础医学院神经科学研究所,Email:jingyh@lzu.edu.cn【摘要】目的：通过采用双侧颈总动脉阻断的方法制作脑缺血再灌注动物模型，研究雷帕霉素对大鼠脑缺血再灌注损伤是否冇保护效应。方法：用双侧颈总动脉阻断结扎法制作缺血模型，缺血前侧脑室给药，于缺血20min后再灌
2、注，24h后心脏灌注固定，取脑做连续冠状冰冻切片，光镜下观察拍照，无偏差计数法计算细胞密度。结果：统计结呆显示假手术组和缺血组的分子层细胞较少，而锥体细胞层的细胞较多；从前额皮层和额叶来看，颛叶的细胞密度较低，而前额皮层的细胞密度较高；给予Rapa,可以看出，缺血前12h给药组的细胞数较多，而缺血前30min给药组的细胞较少。由此可得损伤主要在颍叶的分子层；缺血前12h给雷帕霉索的效果优于缺血前30min给雷帕霉索。结论：雷帕霉索对大鼠脑缺血再灌注损伤有保护效应。【关键词】雷帕霉素；脑缺血；再灌注损伤；保护Toinvestigatetheprot
3、ectiveeffectofRapamycinoncerebralischemia-reperfusioninjuryinrats.WANGLijun,WUDengyan,HUAnchun,YANGCheng,JINGYuhong.1.SchoolofBasicMedicalSciences,LanzhouUniversity,Lanzhou730000,Gansu,China・2.InstituteofNeuroscience,LabofNeurodegenerativedisease,LanzhouUniversity,Lanzhou7300
4、00,Gansu,China.Fundedby:UniversityStudents'InnovationandVenturePlanofLanzhouUniversity.^Correspondingauthor:JINGYuhong,doctor,associateprofessor,InstituteofNeuroscience,LabofNeurodegenerativedisease,LanzhouUniversity,Email:jingyh@lzu・edu.cn（Abstract）Objective:throughtheuseofb
5、ilateralcarotidarteryocclusionmethodproducedanimalmodelsofcerebralischemiatostudytherapamycinoncerebralischemia-reperfusioninjuryhasprotectiveeffects.Methods:Withbilateralcarotidarteryocclusionmodelofischemiabyligation,intracerebroventricularadministrationbeforeischemia,reper
6、fusionafter20minischemia,after24h,perfusionandfixation,andthebraintodoconsecutivecoronalfrozensectionsobservedunderlightmicroscopepictures,unbiasedcountingmethodbasedoncelldensity.Results:Thestatisticsindicatedthat,comparedwiththepyramidalcelllayer,thecellsofthemolecularlayer
7、intheshamgroupandischemiagroupareless;fromthepointofview,prefrontalcortexandtemporallobe,thecelldensityofthetemporallobeislower,whilethecelldensityofprefrontalcortexismuchhigher;givingrapamycin,wecanseethat12hbeforeischemiahasmorecellsthan30minbeforeischemia・Inaword,theinjury
8、ismainlyinthemolecularlayerofthetemporallobe;andcomparedwithdosingat
