毕业设计论文：乙酰丙酸论文

《毕业设计论文：乙酰丙酸论文》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、酱油中乙酰丙酸测定方法研究摘要由于生产工艺的不同，酱油可分为酿造酱油、水解酱油和配制酱油三种。这三种酱油的生产成本及营养价值差异很大，其屮酿造酱油营养价值及生产成本最高，为有效防止其他种类的酱油冒充酿造酱油，保护消费者的利益，急需建立冇效的可操作性的分析方法。酿造酱油屮乙酰丙酸含量极微，I佃英他酱油屮乙酰丙酸含量相对较高，所以酱油屮乙酰丙酸的含量是区分酿造酱油和其他酱油的重耍指标Z-O本文采用高效液相色谱法研究了酱油屮乙酰丙酸含量的测定方法，结果表明：当色谱条件为Lichrspher-Cis(150m

2、mX4.6mm,i.d.5

3、im)，流动相甲醇:水=15:85，流速1.OmL/min,检测波长266nm,进样体积5pL,釆用了标准加入法定量，乙酰丙酸加入量与英峰而积在5.00〜25.00mg-mL-1范围内冇良好的线性关系，方法的重现性(RSD)为2.3%。经用于实际样品分析，在酿造酱油屮，其相关系数r为0.9995,平均加标回收率为99.1%；在配制酱油中，其相关系数r为0.9996，平均加标回收率为98.5%。此法操作简单、快速，符合要求。关键词：乙酰丙酸，高效液相色谱法，酱油Studyon

4、theanalysismethodofLevulinicAcidinsoysauceAbstractSoysauceisconsistoftheblendedsoysauces,thehydrolyticsoysauceandfermentedsoysaucebecauseofdi氓renttechniqueofproduction.Thesethreesoysaucesdiffergreatlyinproductioncostandnutritivevalue,andthefermentedsoys

5、aucehasthehighestproductioncostandnutritivevalue.Inordertoavoidothersoysaucespersonatingthefennentedsoysauce,thereisanurgentneedtoestablisheffectiveandpracticablemethodtoprotectthebenefitofconsumers.Thecontentoflevulinicacidisrelativelylowerintheferment

6、edsoysauceandhigherinothersoysauces.Sothecontentoflevulinicacidinthesoysaucesisanimportantindextodistinguishthefermentedsoysaucesandothersoysauces-ThedeterminationoflevulinicacidinsoysaucehasbeenstudiedbymeansofHighPerformanceLiquidChromatography(HPLC).

7、Theresultsindicatethatanalyticalconditionsincludecolumn:Ci8(150x4・6mm，5ym),mobilephase:methanol-water(15:85v/v),UVdetectionwavelength:266nm,flowrate:l.OmL/min,oventemperature:25°C.injectionvolume:5(iL.Thestandardadditioncalibrationtechniqueisappliedinth

8、epaper.Therearegoodlinearcorrelationsbetweentheconcentrationsandpeakareasofthecomponents,thelinearrangeisfrom5.00mg・mL‘to25.00mg-mLJ.Thereproducibilityofthecontentoflevulinicacidis2.3%・Thismethodhasbeenappliedtotheanalysisofrealsamples.Theresultsshowtha

9、tthelinearcorrelationcoefficientis0.9995inthefermentedsoysauceand0.9996intheblendedsoysauce,andtheaveragerecoveryis99.1%inthefennentedsoysauceand98.5%intheblendedsoysauce.Theexperimentalresultsshowthatthemethodiseasyandquick.Andt