2 The basic shape and fine structure of

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1、2ThebasicshapeandfinestructureoforganellesObjectiveTofamiliarizewiththeshapeanddistributionoforganellesunderlightmicroscopeToknowtheprincipleandmethodsofdyeinglivingcellsTolearntodrawpicturesofspecimensundermicroscopematerialsLightmicroscope,glassslide,coverslip,lenspaperCedaroil,Xylene,Iodinesolu

2、tion,ObservethefollowingslidesundermicroscopePreparedslideofconvolutedtubuleofratkidney/线粒体Preparedcross-sectionofrabbitspinalganglion/高尔基体Preparedsectionofliver/肝糖procedure1MitochondriaObservepreparedslideofkidneyunder10*lens,youcanseetheroundconvolutedtubuleofkidneywithalayerofepithelialcells,ch

3、angeto40*lensandobservethedarkbluegranulesorshortbarsinthecytoplasmwhicharemitochondria.2golgicomplexObservethepreparedslideofrabbitspinalganglionunder10*lensandyoucanseetheyellowroundnervecells,changeto40*lens,therearemanyblackgranulesorirregularlongnarrowpiecesaroundthenucleuswhicharegolgibodies

4、.3glycogenObservethepreparedslideofliverwith10*lens,changeto40*lensandfindthereddotsinthelivercytoplasmwhichistheglycogen.It’stheenergystoreplaceinlivercell.SizeofOnionCellsPrepareawetmountoffreshonionandstainitwithIodinesolution.Trytogetthethinnestpiecethatyoucan;itshouldbethinnerthanpaper.Itmigh

5、thelpifyoutakeathickpieceandbendituntilitsnapsintotwopieces.Afteritsnaps,thereisusuallyaverythinconnectionremainingbetweenthetwopieces.Thisthinconnectinglayercanbepeeledoffandplacedonamicroscopeslideforviewing.Drawseveralonioncellsastheyappearunderhighpower.