《动物遗传学AnimalGenetics》英文版配套习题试题chapter9

《《动物遗传学AnimalGenetics》英文版配套习题试题chapter9》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、Chapter9QUESTIONSANDPROBLEMS9丄Multiplechoice1)WhenDNAcutwitharestrictionenzyme,theresultingfragmentshaveA)a5’phosphateB)a5ZhgroxylC)a3ZhgroxylD)BothAandCE)bothBandC2)AplasmidusedasaclonigvectorinEcloimusthaveA)anorisequenceB)aselectablemarkerC)UniquerestrictionsiteD)alloftheabo

2、veE)BandConly3)BacterialprotectingtheirownDNAfromrestrictionenzymedamagebyaddinggrouptocertainnucelotidesA)methylB)hydroxylC)phosphateD)amino4)aPolylinkerregioninacloningplasmidischaracterizedbyA)multiplemethylgroupsB)multiplerestrictioncutsitesC)multiplereportergenesD)multiple

3、expressionvectors5)InDNAelectrophoresisfragmentseparationisbasedonA)fragmentsizeC)fragmentchargeB)fragmentnucleotidecontentD)fragmentsequence9.2Matchings1)Restrictionenzyme2)Probe3)Plasmid4)YAC5)DideoxynucleotidesA)OligonucleotidelabeledwithradioactivemarkerB)MoleculesusedinDNA

4、sequencingC)EndonucleasesisolatedfrombacteriaD)SmallcircularfragmentofDNAE)Eukaryoticcloningvector9.3AnendonucleasecalledAvril(na-v-r-twoH)cutsDNAwheneveritfindsthesequence5-CGTAGC-313,-GCATCG-5,.AbouthowmanycutswouldAvrilmakeinthehumangenome,whichisabout3x109basepairslongandab

5、out40%GC?9.4Anewrestrictionendonucleaseisisolatedfromabacterium・ThisenzymecutsDNAintofragmentsthataverage4,096basepairslong.Likeallotherknownrestrictionenzymes,thenewonerecognizesasequenceinDNAthathastwofoldrotationalsymmetry.Fromtheinformationgiven,howmanybasepairsofDNAconstit

6、utetherecognitionsequenceforthenewenzyme?9.5WhatisacDNAlibraryandfromwhatcellulargeneticmaterialisitderived?HowisacDNAlibraryusedincloningparticulargenes?9.6Supposeyouwantedtoproducehumaninsulin(apeptidehormone)bycloning.Assumethatthiscouldbedonebyinsertingthehumaninsulingenein

7、toabacterialhost,where,giventheappropriateconditions,thehumangenewouldbetranscribedthentranslatedintohumaninsulin.Whichdoyouthinkitwouldbebesttouseasyoursourceofthegene,humangenomicinsulinDNAoracDNAcopyofthisgene?Explainyourchoice.9.2Youaregivenagenomiclibraryofyeastpreparedina

8、bacterialplasmidvector.YouarealsogivenaclonedcDNAforhu