《动物遗传学AnimalGenetics》英文版配套习题试题chapter6

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1、Chapter6QUESTIONSANDPROBLEMS6.1Matching,pleaseselectthebestmatchforeachterm1)Ffactor2)TransformationA)thetransferofgenesfromonebacteriumtoanotherbyphagevectorsB)Aself-replicating,circularDNAdistinctfromthemainbacterialchromosome3)Transduction4)ConjugationC)Unidirectionaltransferofextracell

2、ularDNAintocellsD)Unidirectionaltransferofgeneticinformationthroughdirectcellularcontactbetweenadonorbacterialcellandarecipientbacterialcell5)PlasmidE)Asexfactorplasmidcontainedindonorbacterialcellsthatallowsmating6.2IfanE.coliauxotrophAcouldgrowonlyonamediumcontainingthymine,andanauxotrop

3、hBcouldgrowonlyonamediumcontainingleucine,howwouldyoutestwhetherDNAfromAcouldtransformB?6.3InF+xFcrossestheFrecipientisconvertedtoadonorwithveryhighfrequency.However,itisrareforarecipienttobecomeadonorinHfrxF'crosses.Explainwhy.6.4WiththetechniqueofinterruptedmatingfourHfrstrainsweretested

4、forthesequenceinwhichtheytransmittedanumberofdifferentgenestoanFstrain.EachHfrstrainwasfoundtotransmititsgenesinauniquesequence,asshownintheaccompanyingtable(onlythefirstsixgenestransmittedwerescoredforeachstrain).ORDEROFTRANSMISSIONHfrSTRAIN1234FirstOREOFHMGBMHXAERCEACRLastMBXHWhatisthege

5、nesequenceintheoriginalstrainfromwhichtheseHfrstrainsderive?IndicateonyourdiagramtheoriginandpolarityofeachofthefourHfr^s.Indicatewhethereachofthefollowingoccurs/orisacharacteristicofGeneralizedTransduction(GT)SpecializedTransduction(ST)OccursinBoth(B)OccursinNeither(N)6.5a.PhagecarriesDNA

6、ofbacterialorviralDNAorigin,neverboth.b.PhagecamesviralDNAcovalentlylinkedtobacterialDNA.c.Phageintegratesintoaspecificsiteonthehostchromosome.d.Phageintegratesatarandomsiteonthehostchromosome.e."Headful”ofbacterialDNAencoated.f.Hostlysogenized.a.Prophagestateexists.b.Temperatephageinvolve

7、d.c.Virulentphageinvolved.6.6Considerthefollowingtransductiondata:DONORRECIPIENTSELECTEDMARKERUNSELECTEDMARKERaceF*dhlaceFdhl+aceF+dhl34aceF+leuaceFleu+aceF+leuIsdhlorleuclosertoaceF?6.7ConsiderthefollowingdatawithPltransduction:DONORaroApyrD4aroA*cmlBcmlBpyrD