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1、利用T细胞受体变异β基因谱系分析T细胞急性淋巴细胞白血病病人T细胞克隆性中国病理生理杂志2000年第7期第16卷论著作者：李杨秋 陈少华 杨力建 祁明芳关键词：受体，抗原，T细胞；基因；白血病 ［摘 要］ 目的：分析T细胞-急性淋巴细胞白血病(T-ALL)病人的T细胞克隆性。方法：利用RT-PCR方法分析6例T-ALL和10例正常人外周血单个核细胞中24个T细胞受体变异β(TCRVβ)基因的CDR3长度，PCR产物再进一步进行基因扫描和序列分析。结果：3例病人的某些TCRVβ亚家族T细胞呈单克隆或寡克隆性增殖，主要为Vβ2、3、6、9、
2、21和24。其它3例及正常人均表现为多克隆性增殖T细胞。结论：部分T-ALL来自于TCRVβ亚家族克隆性增殖T细胞。该方法有助于临床上检测微小残留病变。 ［中图分类号］ R733.71；R392.11 ［文献标识码］ AAnalysisofTcellclonalitybyusingT-cellreceptorvaribleβgene repertoireinT-cellacutelymphoblasticleukemia·LIYang-qiu,CHENShao-hua,YANGLi-jian,QIMing-fang (Depar
3、tmentofHematology,MedicalCollege,JinanUnivesity,Guangzhou510632，China) ［Abstract］ AIM:ToanalyzeTcellclonalityinpatientswithTcellacutelymphoblasticleukemia(T-ALL).METHODS:Thecomplementaritydeterminingregion3(CDR3)sizeof24Tcellantigenreceptorvariableβ(TCRVβ)regiongenewasan
4、alyzedinperipheralbloodmononuclearcell(PBMC)samplesfrom6T-ALLcasesand10normalindividualsbyusingreversetranscriptase-polymerasechainreaction(RT-PCR).ThePCRproductswerefurtherstudiedbygenescanandsequencinganalysis.RESULTS:SomeTCRVβsubfamilyTcellsdisplaymono-oroligoclonalexp
5、ansionsin3casesofT-ALL,predominantlyinVβ2,Vβ3,Vβ6,Vβ9,Vβ21orVβ24,respectively.PolyclonalexpansionsofTcellswerefoundintheotherthreecases,whichcouldalsobefoundinnormalsamples.CONCLUSION:ApartofTcellacutelymphoblasticleukemiacellsmayarisefromaclonalexpansionofTCRVβsubfamilyT
6、cell.Thismethodmaybeusefulforthedetectionofminimalresidualdiseaseinclinicalstudyofthedisease. ［MeSH］ Receptors,antigen,T-cell;Genes;Leukemia ［CLCnumber］ R733.71;R392.11 ［Documentcode］ A ［ArticleFD］ 1000-4718(2000)07-0627-06 Recently,TcellreceptorVβgenerepertoireandc
7、lonalityhavebeenstudiedinpatientswithleukemiaandsolidtumors,byassayingtheCDR3sizeofTcellreceptor(TCR)geneswiththeuseofRT-PCRandgenescananalysis［1,2］.TCRsareheterodimerscomprisingeitherα/βorγ/δchains.Likeimmunoglobin(Ig),byrearrangementofV(D)J-genesegmentsandrandomlyinsert
8、ednucleotides,thisistermedcomplementaritydeterminingregion3(CDR3)anddisplayshighdiversity［2］.The
