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1、ResearchDefense-relatedtranscriptionfactorsWRKY70andWRKY54modulateosmoticstresstolerancebyregulatingstomatalapertureinArabidopsis121,3141,31JingLi,SebastienBesseau,PetriTor€onen€,NinaSipari,HannesKollist,LiisaHolmandE.TapioPalva12ViikkiBiocenter,Divisi
2、onofGenetics,DepartmentofBiosciences,UniversityofHelsinki,FI-00014,Helsinki,Finland;UniversiteFrancßoisRabelaisdeTours,EA2106Biomoleculeset34BiotechnologiesVegetales,37200,Tours,France;InstituteofBiotechnology,UniversityofHelsinki,FI-00014,Helsinki
3、,Finland;InstituteofTechnology,UniversityofTartu,Nooruse1,Tartu,50411,EstoniaSummaryAuthorforcorrespondence:WRKYtranscriptionfactors(TFs)havebeenmainlyassociatedwithplantdefense,butrecentTapioPalvastudieshavesuggestedadditionalrolesintheregulationofot
4、herphysiologicalprocesses.Tel:+358919159600Here,weexploredthepossiblecontributionoftworelatedgroupIIIWRKYTFs,WRKY70andEmail:tapio.palva@helsinki.fiWRKY54,toosmoticstresstolerance.TheseTFsarepositiveregulatorsofplantdefense,andReceived:17May2013co-operat
5、easnegativeregulatorsofsalicylicacid(SA)biosynthesisandsenescence.Accepted:21May2013Weemployedsingleanddoublemutantsofwrky54andwrky70,aswellasaWRKY70overexpressorline,toexploretheroleoftheseTFsinosmoticstress(polyethyleneglycol)NewPhytologist(2013)200
6、:457472responses.Theireffectongeneexpressionwascharacterizedbymicroarraysandverifiedbydoi:10.1111/nph.12378quantitativePCR.Stomatalphenotypeswereassessedbywaterretentionandstomatalcon-ductancemeasurements.Thewrky54wrky70doublemutantsexhibitedclearlyenh
7、ancedtolerancetoosmoticstress.Keywords:abscisicacid(ABA),Arabidopsis,generegulation,osmoticstress,salicylicacidHowever,geneexpressionanalysisshowedreducedinductionofosmoticstress-responsive(SA),stomata,WRKYtranscriptionfactor.genesinadditiontoreducedac
8、cumulationoftheosmoprotectantproline.Bycontrast,theenhancedtolerancewascorrelatedwithimprovedwaterretentionandenhancedstomatalclosure.ThesefindingsdemonstratethatWRKY70andWRKY54co-operateasnegativeregulatorsofstomatalclosureand,conseque
