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1、Downloadedfromhttp://rsif.royalsocietypublishing.org/onAugust11,2018J.R.Soc.Interfacedoi:10.1098/rsif.2012.0271PublishedonlineThesensitivityofhumanmesenchymalstemcellstovibrationandcoldstorageconditionsrepresentativeofcoldtransportationN.I.Nikolaev,Y.Liu*,H.H
2、usseinandD.J.WilliamsCentreforBiologicalEngineering,WolfsonSchoolofMechanicalandManufacturingEngineering,LoughboroughUniversity,Loughborough,LeicestershireLE113TU,UKInthecurrentstudy,themechanicalandhypothermicdamageinducedbyvibrationandcoldstorageonhumanmese
3、nchymalstemcells(hMSCs)storedat2–88Cwasquantifiedbymeasur-ingthetotalcellnumberandcellviabilityafterexposuretovibrationat50Hz(peak2222acceleration140msandpeakdisplacement1.4mm),25Hz(peakacceleration140ms,22peakdisplacement5.7mm),10Hz(peakacceleration20ms,peakd
4、isplacement5.1mm)andcoldstorageforseveraldurations.Toquantifytheviabilityofthecells,inadditiontothetrypanblueexclusionmethod,thecombinationofannexinV-FITCandpropidiumiodidewasappliedtounderstandthemodeofcelldeath.Cellgranularityandapanelofcellsurfacemarkersfo
5、rstemness,includingCD29,CD44,CD105andCD166,werealsoevaluatedforeachcondition.ItwasfoundthathMSCsweresensitivetovibrationat25Hz,withmoder-ateeffectsat50Hzandnoeffectsat10Hz.Vibrationat25HzalsoincreasedCD29andCD44expression.Thestudyfurthershowedthatcoldstoragea
6、lonecausedadecreaseincellviability,especiallyafter48h,andalsoincreasedCD29andCD44andattenuatedCD105expressions.Celldeathwouldmostlikelybetheconsequenceofmembranerupture,owingtonecrosisinducedbycoldstorage.Thesensitivityofcellstodifferentvibrationswithinthemec
7、hanicalsystemisduetoacombinedeffectofdisplacementandacceleration,andhMSCswithalongercoldstoragedurationweremoresusceptibletovibrationdamage,indicatingacouplingbetweentheeffectsofvibrationandcoldstorage.Keywords:stemcells;mechanicalstress;vibration;regenerativ
8、emedicine;hypothermia;viability1.INTRODUCTIONstorage,theprocessofstorageandtransportat2–88Cwassimulated.Recently,challengesfacedduringtheshippingofcellSomelogisticscompanies(forexample,Te
