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1、窗体顶端HightemperaturePEMfuelcells ReviewArticleJournalofPowerSources,Volume160,Issue2,6October2006,Pages872-891JianluZhang,ZhongXie,JiujunZhang,YanghuaTang,ChaojieSong,TitichaiNavessin,ZhiqingShi,DatongSong,HaijiangWang,DavidP.Wilkinson,Zhong-ShengLiu,StevenHoldcroft Showpreview
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4、 Relatedreferenceworkarticles Purchase$41.95969Refinedstructureofc-phycocyaninfromthecyanobacteriumSynechococcusvulcanusat1.6Å:insightsintotheroleofsolventmoleculesinthermalstabilityandco-factorstructure OriginalResearchArticleBiochimicaetBiophysicaActa(BBA)-Bioenergetics,Volume1556,
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8、Figures/TablesFigures/Tables
9、ReferencesReferencesAbstractThecrystalstructureofthelight-harvestingphycobiliprotein,c-phycocyaninfromthethe
10、rmophiliccyanobacteriumSynechococcusvulcanushasbeenrefinedto1.6Åresolutionbasedonthepreviouslydeterminedlowerresolutionstructure(PDBentry1I7Y).Theimproveddatawascollectedusingsynchrotronradiationat100K.Thesignificantlyimprovedcrystallographicdatahasleadtoimprovedcalculatedelectrondensitymaps,al
11、lowingtheunambiguouspositioningofallproteinandco-factoratomsandthepositioningof377solventmolecules.Thepositionsofsolventmoleculesatspecificsitesimportantforstabilizationofdifferentlevelsofself-assemblyofthephycobilisomestructurewereidentifiedandthebondingnetworkisdescribed.Thepresenceofsolventm
12、oleculesinthevicinityoftheco-factorsandinintermolecularspacesisidentifiedandtheirPurchase$41.95possiblerolesaresuggested.Allthreeofthephycocyanobilinco-factorsbindwatermoleculesatspecificsitesbetweenthepropionicacidsidechains.Moleculardynamic(MD)simulationssupportthatthesespecialwatershavearole
13、instabilizationofthisconformation.Onthebasisofthecrystalpackingreportedhereandincomparisontootherphycobiliproteincrystalforms,wehaveanalyzedtherolesofspecificsitesontheformationofthephycobilisomecomplex.ArticleOutline1.Introductio
