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1、THEJOURNALOFBIOLOGICALCHEMISTRYVOL.287,NO.3,pp.1679–1687,January13,2012©2012byTheAmericanSocietyforBiochemistryandMolecularBiology,Inc.PublishedintheU.S.A.RoleofJunctinProteinInteractionsinCellularDynamicsofCalsequestrinPolymeruponCalciumPerturbation*□SReceivedforpublication,April22,2011,and
2、inrevisedform,November24,2011Published,JBCPapersinPress,November28,2011,DOI10.1074/jbc.M111.254045‡1§1‡‡‡‡KeunWooLee,Jin-SooMaeng,JeongYiChoi,YuRanLee,ChaeYoungHwang,SungSupPark,¶¶‡‡§§HyunKyuPark,BongHyunChung,Seung-GooLee,Yeon-SooKim**,HyesungJeon,SooHyunEom,¶¶§§‡2ChulHeeKang,DoHanKim,andK
3、i-SunKwon‡¶FromtheLaboratoryofCellSignaling,AgingResearchCenter,Bio-NanotechnologyResearchCenter,andIndustrialBiotechnologyandBioenergyResearchCenter,KoreaResearchInstituteofBioscienceandBiotechnology(KRIBB),§Daejeon305-806,Korea,theBio-NanotechnologyResearchCenter,KoreaFoodResearchInstitut
4、e(KFRI),Gyeonggi-Do463-746,Korea,the**DepartmentofSmartFoodsandDrugsandIndangInstituteofMolecularBiology,InjeUniversity,Seoul100-032,‡‡§§Korea,theBiomedicalResearchCenter,KoreaInstituteofScienceandTechnology,Seoul136-791,Korea,theSchoolofLife¶¶Sciences,GwangjuInstituteofScienceandTechnology,
5、Gwangju500-712,Korea,andtheDepartmentofChemistry,WashingtonStateUniversity,Pullman,Washington99163Background:Invitrostudieshavereportedreversiblecalsequestrinpolymerizationanddepolymerization.2Results:LivecellimaginganalysisrevealedCa-dependentdecondensationofcalsequestrinspeckles,consisten
6、twithinvitromicroscopicdata.DownloadedfromConclusion:Calsequestrindepolymerizationbycalciumdepletionrequirescoexistenceofjunctin.Significance:Theroleofcalsequestrininintracellularcalciumhomeostasiswasexplored.Calsequestrin(CSQ),themajorintrasarcoplasmicreticulumtor(RyR),formsalargemacromolec
7、ularcomplexwithtrans-http://www.jbc.org/calciumstorageprotein,undergoesdynamicpolymerizationmembrane,luminal,andcytoplasmiccomponents(1).RyR2anddepolymerizationinaCa-dependentmanner.However,recruitsalowaffinity(Kd1mM),highcapacity(60–80molnodirec
