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1、ViewArticleOnline/JournalHomepage/TableofContentsforthisissueCRITICALREVIEWwww.rsc.org/analystAnalystAnalyticalstrategiesfordetectingnanoparticleproteininteractionsaabbabLiwenLi,QingxinMu,BinZhangandBingYanReceived8thFebruary2010,Accepted25thMarch2010FirstpublishedasanAdvance
2、Articleontheweb26thMay2010DOI:10.1039/c0an00075bAsignificantincreaseinbiomedicalapplicationsofnanomaterialsandtheirpotentialtoxicitydemandsversatileanalyticaltechniquestodetermineprotein–nanoparticle(NP)interactions.Thesediverseanalyticaltechniquesarereviewed.Spectroscopicmeth
3、odsplayasignificantroleinstudyingbindingaffinity,bindingratio,andbindingmechanisms.ToelucidateNP–proteomeinteractions,chromatographyandelectrophoresistechniquesareappliedtoseparateNP-boundproteinsandmatrixassistedlaserdesorptionionizationtime-of-flightmassspectrometry(MALDI-TOF-
4、MS)toidentifytheseproteins.SinceNP–proteinbindingisadynamicevent,surfaceplasmonresonance(SPR)andquartzcrystalmicrobalance(QCM)aremethodsofchoicetostudythekineticsofNP–proteinbinding.1.Introductiontoalteredproteinconformationandtheexposureofnovelepitopes.10Theperturbedsignalin
5、gtransductionincellsmayDuetotheuniquepropertiesatthenanoscale,nanoparticleshaveadverseeffectsoncellularfunctionandcausetoxicity(NPs)arebeingdevelopedasdrugcarriers,1,2imagingagentsforinvitroandinvivo.cancer,3–5aswellasanalyticalprobes.6,7WithmoreandmoreTherefore,analyticalmet
6、hodsandstrategiesareessentialtoapplicationsofnanotechnologyandthereleaseofnano-investigateNP–proteininteractionsinordertounderstandthematerialsintotheenvironment,therearealsoincreasingsocietalmechanisticbasisforthebiologicalactivityoftheNPandtoconcernsaboutnanosafety.8,9Foral
7、argepart,bothpositivemakesafeuseofnanotechnology.Herein,wesummarizerecentandnegativesidesofnanoparticlesarerelatedtotheirdramaticprogressesonthedevelopmentofanalyticalstrategiestoinves-biologicalmembranepermeabilityandtheirstronginteractionstigateNP–proteininteractions.Wefocu
8、sonthefunctionalrolewithbiomacromolecules,suchasproteins.Investigati
