Aphid Resistance in Medicago truncatula Involves Antixenosis and Phloem-Specific, Inducible Antibiosis, and Maps to a Single Locus Flanked by NBS-LRR Resistance Gene Analogs英文资料

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1、AphidResistanceinMedicagotruncatulaInvolvesAntixenosisandPhloem-Specific,InducibleAntibiosis,andMapstoaSingleLocusFlankedbyNBS-LRR1ResistanceGeneAnalogsJohnKlingler,RobertCreasy,LinglingGao,RamakrishnanM.Nair,AlonsoSuazoCalix,HelenSpaffordJacob,OwainR.Edwards,

2、andKaramB.Singh*CommonwealthScientificandIndustrialResearchOrganizationEntomology(J.K.,L.G.,O.R.E.)andCommonwealthScientificandIndustrialResearchOrganizationPlantIndustry(J.K.,L.G.,A.S.C.,K.B.S.),Wembley,WesternAustralia6913,Australia;SchoolofAnimalBiology,Univ

3、ersityofWesternAustralia,Crawley,WesternAustralia6009,Australia(R.C.,H.S.J.);andSouthAustralianResearchandDevelopmentInstitute,Adelaide,SouthAustralia5001,Australia(R.M.N.)Aphidsandrelatedinsectsfeedfromasinglecelltypeinplants:thephloemsieveelement.Geneticres

4、istancetoAcyrthosiphonkondoiShinji(bluegreenaphidorbluealfalfaaphid)hasbeenidentifiedinMedicagotruncatulaGaert.(barrelmedic)andbackcrossedintosusceptiblecultivars.ThestatusofM.truncatulaasamodellegumeallowsanin-depthstudyofdefenseagainstthisaphidatphysiologica

5、l,biochemical,andmolecularlevels.Inthisstudy,twocloselyrelatedresistantandsusceptiblegenotypeswereusedtocharacterizetheaphid-resistancephenotype.Resistanceconditionsantixenosissincemigratoryaphidsweredeterredfromsettlingonresistantplantswithin6hofrelease,pref

6、erringtosettleonsusceptibleplants.AnalysisoffeedingbehaviorrevealedthetraitaffectsA.kondoiatthelevelofthephloemsieveelement.Aphidreproductiononexcisedshootsdemonstratedthatresistancerequiresanintactplant.AntibiosisagainstA.kondoiisenhancedbypriorinfestation,i

7、ndicatinginductionofthisphloem-specificdefense.Resistancesegregatesasasingledominantgene,AKR(Acyrthosiphonkondoiresistance),intwomappingpopulations,whichhavebeenusedtomapthelocustoaregionflankedbyresistancegeneanalogspredictedtoencodetheCC-NBS-LRRsubfamilyofres

8、istanceproteins.Thisworkprovidesthebasisforfuturemolecularanalysisofdefenseagainstphloemparasitisminaplantmodelsystem.Parasitismbyphloem-feedinginsects,suchasaphidsedgeofplant-microbeinte