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1、EMBRYONICSTEMCELLS:CHARACTERIZATIONSERIESQualificationofEmbryonalCarcinoma2102EpAsaReferenceforHumanEmbryonicStemCellResearchabcccRICHARDJOSEPHSON,CAROLJ.ORDING,YINGLIU,SOOJUNGSHIN,UMALAKSHMIPATHY,accdcARAZTOUMADJE,BRADLEYLOVE,JONATHAND.CHESNUT,PETERW.ANDREWS,MAHENDRAS.RAO,aJONATHANM.AUERBACHabGlob
2、alStem,Inc.,Rockville,Maryland,USA;StemCellCenter,AmericanTypeCultureCollection,Manassas,cdVirginia,USA;CorporateResearchLaboratories,InvitrogenCorporation,Carlsbad,California,USA;CentreforStemCellBiologyandDepartmentofBiomedicalScience,TheUniversityofSheffield,Sheffield,UnitedKingdomKeyWords.Embry
3、onicstemcells•Embryonalcarcinoma•Standard•Pluripotent•HumanABSTRACTAsthenumberofhumanembryonicstemcell(hESC)linesshouldbewidelyavailablewithoutintellectualpropertyre-increases,sodoestheneedforsystematicevaluationofeachstraints,easilyculturedwithoutfeeders,andresistanttoline’scharacteristicsandpoten
4、tial.Comparisonsbetweenspontaneouschangesinphenotype.Weproposethatthelinesarecomplicatedbyvariationsincultureconditions,embryonalcarcinoma(EC)line2102Epmeetstheserequire-feeders,spontaneousdifferentiation,andtheabsenceofments.Wecomparedtheprotein,gene,andmicroRNAex-standardizedassays.Thesedifficult
5、ies,combinedwiththepressionofthiscelllinewiththoseofhESClinesandalter-inabilityofmostlabstomaintainmorethanafewlinesnativereferencelinessuchastheEClineNTERA-2andthesimultaneously,compelthedevelopmentofreferencestan-karyotypicallyabnormalhESClineBG01V.TheoveralldardstowhichhESClinescanbecompared.The
6、useofaexpressionprofilesofalltheselinesweresimilar,withex-stablecelllineasareferencestandardoffersmanyadvan-ceptionsreflectingthegermcelloriginsofEC.Onthebasistages.AlinewitharelativelyunchanginghESC-likegeneofglobalgeneandmicroRNAexpression,2102Epissome-andproteinexpressionpatterncouldbeapositivec
7、ontrolwhatlesssimilartohESCthanthealternatives;however,fordevelopingassays.Itcanbeusedasareferencefor2102EpexpressesmorehESC-associatedmicroRNAsthangenomicsorproteomicsstudies,especiallyfornormalizingNTERA-
