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《Functional Analysis of the Epidermal-Specific MYB GenesCAPRICE and WEREWOLF in Arabidopsis W表皮特异性MYB基因的功能分析 拟南芥中的反复无常和狼人》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、ThePlantCell,Vol.19:2264–2277,July2007,www.plantcell.orgª2007AmericanSocietyofPlantBiologistsFunctionalAnalysisoftheEpidermal-SpecificMYBGenesCAPRICEandWEREWOLFinArabidopsisWRumiTominaga,aMinekoIwata,aKiyotakaOkada,a,bandTakujiWadaa,1aPlantScienceCente
2、r,RIKEN,Tsurumi-ku,Yokohama,Kanagawa230-0045,JapanbDepartmentofBotany,GraduateSchoolofScience,KyotoUniversity,KitashirakawaOiwake-cho,Sakyo-ku,Kyoto606-8502,JapanEpidermiscelldifferentiationinArabidopsisthalianaisamodelsystemforunderstandingthedevelop
3、mentalendstateofplantcells.TwotypesofMYBtranscriptionfactors,R2R3-MYBandR3-MYB,areinvolvedincellfatedetermination.Toexaminethemolecularbasisofthisprocess,weanalyzedthefunctionalrelationshipoftheR2R3-typeMYBgeneWEREWOLF(WER)andtheR3-typeMYBgeneCAPRICE(
4、CPC).ChimericconstructsmadefromtheR3MYBregionsofWERandCPCusedinreciprocalcomplementationexperimentsshowedthattheCPCR3regioncannotfunctionallysubstitutefortheWERR3regioninthedifferentiationofhairlesscells.However,WERR3cansubstantiallysubstituteforCPCR3
5、.TherearenodifferencesinyeastinteractionassaysofWERorWERchimeraproteinswithGLABRA3(GL3)orENHANCEROFGLABRA3(EGL3).CPCandCPCchimeraproteinsalsohavesimilaractivityinpreventingGL3WERandEGL3WERinteractions.Furthermore,weshowedbygelmobilityshiftassaysthatWE
6、RchimeraproteinsdonotbindtotheGL2promoterregion.However,aCPCchimeraprotein,whichharborstheWERR3motif,stillbindstotheGL2promoterregion.INTRODUCTIONtouchingonlyonecorticalcelldevelopintohairlesscells.Wild-typeArabidopsishaseighthaircellfilesalignedlongit
7、udinallyCellfatedeterminationisacriticalstepinthedevelopmentalalongtheroot.processesofplantsandinvolvestheparticipationofalargeFrompreviousworkitisclearthattwotypesofMYB-relatednumberoftranscriptionfactors.TheMYBfamilyisoneofthetranscriptionfactors(R2
8、R3andR3)areinvolvedinepidermisdif-largestgroupsoftranscriptionfactorsintheArabidopsisthalianaferentiation.ThecloselyrelatedWEREWOLF(WER),GLABRA1genome,with>125members(Kranzetal.,1998;Strackeetal.,(GL1),andMYB23genesencodeR2R3-typeMYBgenes.WER2
