化学专业英语文献翻译

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1、英语文献翻译学院：化学化工学院班级：应化1106姓名：辛友伶吴海军学号：2011022223420110222227QuantifyingtheClusterofDifferentiation4ReceptorDensityonHumanTLymphocytesUsingMultipleReactionMonitoringMassSpectrometry多反应监测质量光谱法应用于人类T淋巴细胞量化分化抗原簇4受体密度ABSTRACT:Clusterofdifferentiation4(CD4)isanimportantglycoproteincontainingfou

2、rextracellulardomains,atransmembraneportionandashortintracellulartail.ItlocatesonthesurfaceofvarioustypesofimmunecellsandperformsacriticalroleinmultiplecellularfunctionssuchassignalamplificationandactivationofTcells.Itiswell-knownasaclinicalcellsurfaceproteinmarkerforstudyofHIVprogressi

3、onandfordefiningtheThelpercellpopulationinimmunologicalapplications.Moreover,CD4proteinhasbeenusedasabiologicalcalibratorforquantificationofothersurfaceandintracellularproteins.However,flowcytometry,theconventionalmethodofquantificationoftheCD4densityontheTcellsurfacedependsonantibodies

4、andhassufferedfromvariablessuchasantibodyclones,thefluorophoreandconjugationchemistries,thefixationconditions,andtheflowcytometricquantificationmethodsused.Inthisstudy,wereportthedevelopmentofahighlyreproduciblenanoliquidchromatography−multiplereactionmonitoringmassspectrometry-basedqua

5、ntitativemethodtoquantifytheCD4receptordensityinunitsofcopynumberpercellonhumanCD4+Tcells.Themethodutilizesstableisotope-labeledfull-lengthstandardCD4asaninternalstandardtomeasureendogenousCD4directly,withouttheuseofantibodies.Thedevelopmentofthemassspectrometry-basedapproachofCD4protei

6、nquantificationisimportantasacomplementarystrategytovalidatetheanalysisfromthecytometry-basedconventionalmethod.ItalsoprovidesnewsupportforquantitativeunderstandingandadvancedcharacterizationofCD4onCD4+Tcells.摘要：集群分化4(CD4)是一种重要的糖蛋白，它包含四个胞外区域,横跨膜的部分和短的细胞内尾巴。它位于各种类型免疫细胞的表面，在多种细胞功能中扮演重要角色，

7、像细胞信号放大和激活的T细胞。众所周知的是作为研究艾滋病病程的临床细胞表面蛋白标记和在免疫学应用程序中定义辅助T细胞数量。除此之外，CD4细胞蛋白质也已被用作其他表面和胞内蛋白量化的生物校准器。但是，流式细胞，传统量化CD4T细胞表面密度的方法取决于抗体和并且会受到像抗体克隆、荧光团和结合化学、固定条件以及以前流式细胞定量方法等变量带来的改变。在这项研究中，我们报道一种人类CD4+T细胞中量化CD4受体密度在每个细胞的拷贝数的高度可再生的纳米液相色谱-多反应监测质谱为基础的定量方法的发展。该方法利用稳定同位素标记的全长标准CD4作为内部标准来直接衡量