Scherer-2011-Lipid profiling of l

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1、BiochimicaetBiophysicaActa1811(2011)918–924ContentslistsavailableatScienceDirectBiochimicaetBiophysicaActajournalhomepage:www.elsevier.com/locate/bbalipReviewLipidprofilingoflipoproteinsbyelectrosprayionizationtandemmassspectrometry☆MaxScherer,AlfredBöttcher,GerhardLiebisch⁎InstituteofClinicalC

2、hemistryandLaboratoryMedicine,UniversityofRegensburg,GermanyarticleinfoabstractArticlehistory:Lipoproteinsareoffundamentalimportanceforthelipidtransportandcardiovasculardisease.ThefunctionReceived17January2011andmetabolismoflipoproteinsisintimatelylinkedtothebiophysicalpropertiesoftheirsurface

3、lipids.Receivedinrevisedform30May2011Althoughanumberofdiseaseassociationswerefoundforlipidspeciesinplasma,onlyafewstudiesreportedAccepted14June2011lipidprofilesoflipoproteins.Here,weprovideanoverviewoftechniquesforlipoproteinseparation,methodsAvailableonline26June2011forlipidspeciesanalysisbase

4、donelectrosprayionizationtandemmassspectrometry(ESI-MS/MS)aswellasdatafromrecentlipidomicstudiesonlipoproteinfractions.WealsodiscussthedifferentanalyticalstrategiesKeywords:Lipoproteinseparationandhowlipidprofilingcanexpandourunderstandingofthebiologyandstructuresoflipoproteins.ThisLipidomicsar

5、ticleispartofaSpecialIssueentitledLipodomicsandImagingMassSpectrometry.Phospholipids©2011ElsevierB.V.Allrightsreserved.SphingolipidsLipidspecies1.Introductionlipidspecies,ananalysisofthelipoproteinsaslipidcarriersmaybenecessary.Theantiatherogenicfunctionofhighdensitylipoprotein(HDL)[1]Anearlye

6、ventinatherosclerosisistheretentionoflipoproteinsandproatherogenicroleofapolipoproteinBcontaininglipoproteinswithinthearterialwallpromotedbyenzymeslikelipoproteinlipase,[2]iswelldocumented.Untilnow,moststudiesofthelipoproteinsecretorysphingomyelinase,andsecretoryphospholipaseA2[2].Anlipidcompo

7、sitionarelimitedtotheirmajorlipidclassessuchasincreasedsphingomyelin(SM)tophosphatidylcholine(PC)ratiocholesterol,cholesterylester,triglycerides(TG)aswellasthemainmakeslowdensitylipoprotein(LDL)moresusceptibletosecretoryglycerophospholi