Regulation of NAD(+)-Dependent isocitrate dehydrogenase in the citrate producing yeast Yarrowia lipolytica

《Regulation of NAD(+)-Dependent isocitrate dehydrogenase in the citrate producing yeast Yarrowia lipolytica》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、Biochemistry(Moscow),Vol.69,No.12,2004,pp.1391
1398.TranslatedfromBiokhimiya,Vol.69,No.12,2004,pp.1706
1714.OriginalRussianTextCopyright©2004byMorgunov,Solodovnikova,Sharyshev,Kamzolova,Finogenova.+RegulationofNAD
DependentIsocitrateDehydrogenaseintheCitrateProducingYeastYarrowialipolyti

2、caI.G.Morgunov*,N.Yu.Solodovnikova,A.A.Sharyshev,S.V.Kamzolova,andT.V.FinogenovaSkryabinInstituteofBiochemistryandPhysiologyofMicroorganisms,RussianAcademyofSciences,142290Pushchino,MoscowRegion,Russia;fax:(7
095)956
3370;E
mail:morgunovs@rambler.ruReceivedDecember22,2003Revisionreceived

3、March16,2004Abstract—Themechanismoftheincreasedaccumulation(overproduction)ofcitricacidsintheyeastYarrowialipolyticawhilegrowinginthepresenceofglucoseundernitrogendeficiencywasinvestigated.Thelimitationoftheyeastgrowthbythesource++ofnitrogendecreasesthetotalcontentofnucleotidesandincreas

4、estheratiosofATP/AMPandNADH/NAD.NAD
Dependentisocitratedehydrogenase,anenzymeofthetricarboxylicacidcycleplayingakeyroleintheregulationofbiosyn
thesisofcitricandisocitricacids,wasisolatedfromY.lipolytica.Themolecularweightsofthenativeenzymeanditssubunitswerefoundtobe412and52kD,respectivel

5、y.Itisconcludedthattheenzymeisahomooligomerconsistingofeightsubunits.Investigationoftheeffectofsomeintermediatesofthetricarboxylicacidcycleontheactivityofthisenzymesuggeststhatthe+enhancedexcretionofcitricacidscanbecausedbytheinhibitionofNAD
dependentisocitratedehydrogenaseduetothe+decre

6、aseinthecontentofAMPandincreaseintheNADH/NADratiointhecellsofY.lipolyticaunderdepletionofnitro
gen.Keywords:biosynthesisofcitricacids,Yarrowialipolytica,isocitratedehydrogenase,regulationGrowthlimitationoftheyeastYarrowialipolyticabyvationofY.lipolyticaundertheconditionsofgrowthlim
+thes

7、ourceofnitrogenwithexcesscarbonsourceresultsinitationbythesourceofnitrogen.NAD
IDHwasisolat
intensivesynthesisofcitricandisocitricacids.AfteredfromY.lipolytica,purified,andpartiallycharacterized.depletionofthenitrogeninthestationarygrowthphase,Possiblemechanismsofregulati