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1、FoodMicrobiology28(2011)848e861ContentslistsavailableatScienceDirectFoodMicrobiologyjournalhomepage:www.elsevier.com/locate/fmReviewRecentadvancesinquantitativePCR(qPCR)applicationsinfoodmicrobiologya,*b,cadaFlorencePostollec,HélèneFalentin,SoniaPavan,JérômeCombrisson,DanièleSohie
2、raADRIADéveloppement-UMT08.3PHYSI’Opt,Creac’hGwen,29196Quimper,FrancebINRA,UMR1253,ScienceetTechnologieduLaitetdel’Œuf,65ruedeStBrieuc,35000Rennes,FrancecAgrocampusOuest,UMR1253,ScienceetTechnologieduLaitetdel’Oeuf,35000Rennes,FrancedDanoneResearch,91767PalaiseauCedex,Francearticl
3、einfoabstractArticlehistory:Molecularmethodsarebeingincreasinglyappliedtodetect,quantifyandstudymicrobialpopulationsinReceived17November2010foodorduringfoodprocesses.Amongthesemethods,PCR-basedtechniqueshavebeenthesubjectofReceivedinrevisedformconsiderablefocusandISOguidelineshave
4、beenestablishedforthedetectionoffood-bornepathogens.14February2011Moreparticularly,real-timequantitativePCR(qPCR)isconsideredasamethodofchoiceforthedetectionAccepted21February2011andquantificationofmicroorganisms.OneofitsmajoradvantagesistobefasterthanconventionalAvailableonline1Ap
5、ril2011culture-basedmethods.Itisalsohighlysensitive,specificandenablessimultaneousdetectionofdifferentmicroorganisms.Applicationofreverse-transcription-qPCR(RT-qPCR)tostudypopulationKeywords:dynamicsandactivitiesthroughquantificationofgeneexpressioninfood,bycontrastwiththeuseofQuant
6、itativePCRReverse-transcriptionreal-timePCR(RT-qPCR,isjustbeginning.Providedthatappropriatecontrolsareincludedintheanalyses,qPCRandqPCR)RT-qPCRappeartobehighlyaccurateandreliableforquantificationofgenesandgeneexpression.ThisFoodmicrobiologyreviewaddressessomeimportanttechnicalaspec
7、tstobeconsideredwhenusingthesetechniques.PopulationdynamicsRecentapplicationsofqPCRandRT-qPCRinfoodmicrobiologyaregiven.Someinterestingapplicationssuchasriskanalysisorstudyingtheinfluenceofindustrialprocessesongeneexpressionandmicrobialactivityarereported.Ó2011ElsevierLtd.Allrights
8、reserved.1.Introductionqualitativ