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1、FoodMicrobiology28(2011)848e861ContentslistsavailableatScienceDirectFoodMicrobiologyjournalhomepage:www.elsevier.com/locate/fmReviewRecentadvancesinquantitativePCR(qPCR)applicationsinfoodmicrobiologya,*b,cadaFlorencePostollec,HélèneFalentin,SoniaPavan,J
2、érômeCombrisson,DanièleSohieraADRIADéveloppement-UMT08.3PHYSI’Opt,Creac’hGwen,29196Quimper,FrancebINRA,UMR1253,ScienceetTechnologieduLaitetdel’Œuf,65ruedeStBrieuc,35000Rennes,FrancecAgrocampusOuest,UMR1253,ScienceetTechnologieduLaitetdel’Oeuf,35000Renne
3、s,FrancedDanoneResearch,91767PalaiseauCedex,FrancearticleinfoabstractArticlehistory:Molecularmethodsarebeingincreasinglyappliedtodetect,quantifyandstudymicrobialpopulationsinReceived17November2010foodorduringfoodprocesses.Amongthesemethods,PCR-basedtech
4、niqueshavebeenthesubjectofReceivedinrevisedformconsiderablefocusandISOguidelineshavebeenestablishedforthedetectionoffood-bornepathogens.14February2011Moreparticularly,real-timequantitativePCR(qPCR)isconsideredasamethodofchoiceforthedetectionAccepted21Fe
5、bruary2011andquantificationofmicroorganisms.OneofitsmajoradvantagesistobefasterthanconventionalAvailableonline1April2011culture-basedmethods.Itisalsohighlysensitive,specificandenablessimultaneousdetectionofdifferentmicroorganisms.Applicationofreverse-tran
6、scription-qPCR(RT-qPCR)tostudypopulationKeywords:dynamicsandactivitiesthroughquantificationofgeneexpressioninfood,bycontrastwiththeuseofQuantitativePCRReverse-transcriptionreal-timePCR(RT-qPCR,isjustbeginning.Providedthatappropriatecontrolsareincludedint
7、heanalyses,qPCRandqPCR)RT-qPCRappeartobehighlyaccurateandreliableforquantificationofgenesandgeneexpression.ThisFoodmicrobiologyreviewaddressessomeimportanttechnicalaspectstobeconsideredwhenusingthesetechniques.PopulationdynamicsRecentapplicationsofqPCRan
8、dRT-qPCRinfoodmicrobiologyaregiven.Someinterestingapplicationssuchasriskanalysisorstudyingtheinfluenceofindustrialprocessesongeneexpressionandmicrobialactivityarereported.Ó2011ElsevierLtd.Allrightsreserved.1.Introductionqualitativ
