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1、15-5-4ApplicationofepifluorescencescanningformonitoringtheefficacyofproteinremovalbyRFgas–plasmadecontamination-IOPscienceNewJournalofPhysicsVolume11number11AbstractReferencesNewJ.Phys.11(2009)115028doi:10.1088/1367-2630/11/11/115028Applicationofepifluorescencescanningformonitoringtheefficacyofprot
2、einremovalbyRFgas–plasmadecontaminationHelenCBaxter1,PatriciaRRichardson1,GaynorACampbell1,ValeriIKovalev2,RobertMaier2,JamesSBarton2,AnitaCJones1,GregDeLarge3,MarkCasey4andRobertLBaxter1,51SchoolofChemistry,JosephBlackChemistryBuilding,TheUniversityofEdinburgh,WestMainsRoad,EdinburghEH93JJ,UK2Scho
3、olofEngineeringandPhysicalScience,Heriot-WattUniversity,EdinburghEH144AS,UK3PlasmaEtchInc,3522ArrowheadDrive,CarsonCity,NV89706,USA4SterileServicesDepartment,RoyalInfirmaryofEdinburgh,EdinburghEH164AS,UK5Authortowhomanycorrespondenceshouldbeaddressed.E-mail:r.baxter@ed.ac.ukReceived7April2009Publis
4、hed26November2009Abstract.Thedevelopmentofmethodsformeasuringtheefficiencyofgas–plasmadecontaminationhaslaggedfarbehindapplication.Anapproachtomeasuringtheefficiencyofproteinremovalfromsolidsurfacesusingfluorescein-labelledbovineserumalbuminandepifluorescencescanning(EFSCAN)isdescribed.Amethodforfl
5、uorescentlylabellingproteins,whichareadsorbedanddenaturedonmetalsurfaces,hasbeendeveloped.Bothapproacheshavebeenusedtoevaluatetheefficiencyofradiofrequency(RF)gas–plasmadecontaminationprotocols.Exampleswith`real'surgicalinstrumentsdemonstratethatanargon–oxygenRFgas–plasmatreatmentcanroutinelyreduce
6、theproteinloadbyaboutthreeordersofmagnitudebeyondthatachievedbycurrentdecontaminationmethods.Contents1.Introduction2.Materialsandmethods3.Resultsanddiscussion3.1.MeasuringtheefficiencyofRFgas-plasmadecontamination3.2.Insitulabellingofproteinsonsurfaces3.3.Visualizationofproteincontaminationonsurgic
7、alinstruments4.Conclusionsiopscience.iop.org/1367-2630/11/11/115028/fulltext/1/1415-5-4ApplicationofepifluorescencescanningformonitoringtheefficacyofproteinremovalbyRFgas–plasmadecontamination-IOPscienceAck
