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1、ThePlantJournal(2001)28(2),123±133AnewmemberoftheArabidopsisWRKYtranscriptionfactorfamily,AtWRKY6,isassociatedwithbothsenescence-anddefence-relatedprocesses*SilkeRobatzekandImreE.SomssichMax-Planck-InstitutfuÈrZuÈchtungsforschung,AbteilungBiochemie,Carl-von-Linne-Weg10,50829K
2、oÈln,GermanyReceived21May2001;accepted2July2001.*Forcorrespondence(fax+492215062313;e-mailsomssich@mpiz-koeln.mpg.de).SummaryWRKYproteinsconstitutealargefamilyofplant-speci®ctranscriptionfactorswhoseprecisefunctionshaveyettobeelucidated.Hereweshowthatexpressionofonerepresenta
3、tiveinArabidopsis,AtWRKY6,isin¯uencedbyseveralexternalandinternalsignalsofteninvolvedintriggeringsenescenceprocessesandplantdefenceresponses.Progressive5¢deletionsoftheAtWRKY6promoterallowedseparationofde®nedregionsresponsiblefortheexpressionindistinctorgansoruponpathogenchal
4、lenge.NuclearlocalizationofAtWRKY6wasdemonstrated;proteintruncationsandgain-of-functionstudiesenableddelineationofaregionharbouringanoveltypeoffunctionalnuclearlocalizationsignal(NLS).Keywords:WRKYproteins,leafsenescence,pathogeninfection,promoteranalysis,nucleartargeting.Int
5、roductionWRKYproteinsareplant-speci®ctranscriptionfactorstransactivationcapability(dePateretal.,1996;Eulgemencodedbyamultigenefamilycomprisingover75mem-etal.,1999;Haraetal.,2000).bersinArabidopsis.WRKYfactorswereoriginallyidenti-AlthoughalargenumberofWRKYgenesandEST®edinnumer
6、ousplantsbytheirabilitytobindDNAsequencesarepresentindatabases,littleinformationissequence-speci®cally(Eulgemetal.,2000).Onecommonavailableconcerningtheirspatialexpressionbehaviourorfeatureofthisfamilyisahighlyconservedpeptidestretchtheirregulatoryfunctioninplanta.Inparsley,r
7、iceandof60aminoacidswiththestronglyconservedandname-tobacco,WRKYgeneshavebeendescribedprimarilyasgivingWRKYGQKpeptideinitsN-terminus,andanovelbeingpathogen-inducibleorinducedupontreatmentspotentialC-C-H-H/Czinc-®ngermotifinitsC-terminalpart.withpathogen-derivedelicitorsorsali
8、cylicacid,bywound-PreviousstudiesdemonstratedtheDNA-bindingfunctioni
