链霉菌实验操作手册

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1、链霉菌室实验操作手册（2003年）第一章培养基抗生素生长因子.........................................3常用抗生素及其使用浓度.......................................................................................................3LB（Luria-Bertani）培养基.....................................................

2、.............................................3LA.............................................................................................................................................3基本培养基（MM）.............................................................

3、..................................................3R5（1L）链霉菌原生质体转化时用.....................................................................................4YEME(酵母膏-麦芽膏培养基)1L（液体培养链霉菌）.....................................................4TSBY（1L）（液体培养链霉菌）........

4、................................................................................4MS培养基................................................................................................................................52CMY培养基（用于井岗的接合转移）...........................

5、...................................................5YMS培养基（阿维，井岗产孢）.........................................................................................5YD培养基..................................................................................................

6、..............................5生长因子补充物的使用浓度...................................................................................................5第二章DNA基本操作..............................................................7大肠杆菌质粒的抽提...................................

7、............................................................................7酶连反应...................................................................................................................................7DNA片段凝胶回收......................................

8、...........................................................................7DNA纯化...................................................................................................