资源描述:
《Efficiency of RNA extraction from selected bacteria in the context of biogas production and metatranscriptomics 》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、Anaerobe29(2014)85e90ContentslistsavailableatScienceDirectAnaerobejournalhomepage:www.elsevier.com/locate/anaerobeMolecularbiology,geneticsandbiotechnologyEfficiencyofRNAextractionfromselectedbacteriainthecontextofbiogasproductionandmetatranscriptomicsLucyStark*,TinaGiersch,
2、RöbbeWünschiersFacultyofMathematics,NaturalSciencesandComputerSciences,UniversityofAppliedSciencesMittweida,Technikumplatz17,09648Mittweida,GermanyarticleinfoabstractArticlehistory:UnderstandingthemicrobialpopulationinanaerobicdigestionisanessentialtasktoincreaseefficientRec
3、eived22May2013substrateuseandprocessstability.Themetabolicstate,representede.g.bythetranscriptome,ofafer-Receivedinrevisedformmentingsystemcanhelptofindmarkersformonitoringindustrialbiogasproductiontopreventfailures5September2013ortomodelthewholeprocess.Advancesinnext-genera
4、tionsequencingmaketranscriptomesaccessibleAccepted10September2013forlarge-scaleanalyses.Inordertoanalyzethemetatranscriptomeofamixed-speciessample,isolationAvailableonline4October2013ofhigh-qualityRNAisthefirststep.However,differentextractionmethodsmayyielddifferenteffi-cienc
5、iesindifferentspecies.Especiallyinmixed-speciesenvironmentalsamples,unbiasedisolationofKeywords:transcriptsisimportantformeaningfulconclusions.WeappliedfivedifferentRNA-extractionprotocolsBiogasproductionMetatranscriptomicstoninetaxonomicdiversebacterialspecies.Chosenmethods
6、arebasedonvariouslysisandextractionMixedculturesprinciples.WefoundthattheextractionefficiencyofdifferentmethodsdependsstronglyonthetargetNucleicacidpurificationorganism.RNAisolationofgram-positivebacteriawascharacterizedbylowyieldwhilstfromgram-RNAextractionnegativespecieshig
7、herconcentrationscanbeobtained.Transferringourresultstomixed-speciesin-vestigations,suchasmetatranscriptomicswithbiofilmsorbiogasplants,leadstotheconclusionthatparticularmicroorganismsmightbeover-orunderrepresenteddependingonthemethodapplied.Specialcaremustbetakenwhenusingsu
8、chmetatranscriptomicsdatafor,e.g.processmodeling.Ó2013ElsevierLtd.Allrightsreserve