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1、Downloadedfromwww.genesdev.orgonOctober15,2007Changesinhistonegenedosagealtertranscriptioninyeast.CDClark-Adams,DNorris,MAOsley,JSFasslerandFWinstonGenes&Dev.19882:150-159Accessthemostrecentversionatdoi:10.1101/gad.2.2.150ReferencesThisarticlecites41articles,16ofwhich
2、canbeaccessedfreeat:http://www.genesdev.org#ReferencesArticlecitedin:http://www.genesdev.org/cgi/content/abstract/2/2/150#otherarticlesEmailalertingReceivefreeemailalertswhennewarticlescitethisarticle-signupintheboxattheservicetoprightcornerofthearticleorclickhereNote
3、sTosubscribetoGenesandDevelopmentgoto:http://www.genesdev.org/subscriptions/©1988ColdSpringHarborLaboratoryPressDownloadedfromwww.genesdev.orgonOctober15,2007ChangesinhistonegenedosagealtertranscriptioninyeastChrisD.Clark-Adams,1DavidNorris,2MaryAnnOsley,2,3JanS.Fassl
4、er,1,4andFredWinston11DepartmentofGenetics,HarvardMedicalSchool,Boston,MA02115USA;2Dana-FarberCancerCenter,Boston,MA02115USAandDepartmentofMicrobiologyandMolecularGenetics,HarvardMedicalSchool,Boston,MA02115USAChromatinstructureisbelievedtobeimportantforanumberofcellu
5、larprocesses,includingtranscription.However,theroleofnucleosomesintranscriptionisnotwellunderstood.WehaveidentifiedtheyeasthistonelocusHTB1-HTB1,encodinghistonesH2AandH2B,asasuppressorofsolo8insertionmutationsthatinhibitadjacentgeneexpression.TheHTA1-HTB1locuscausessu
6、ppressioneitherwhenpresentonahigh-copy-numberplasmidorwhenmutant.ThesechangesinHTA1-HTB1altertranscriptionofthegenesadjacenttothe8insertions.Onthebasisofthisresult,wehaveexaminedtheeffectsofincreasedanddecreasedhistonegenedosageforallfouryeasthistoneloci.Fromthetypeso
7、fhistonegenedosagechangesthatcausesuppressionofinsertionmutations,weconcludethatalteredstoichiometryofhistonedimersetscanaltertranscriptioninyeast.[KeyWords:Histones;transcription;geneexpression;yeast]ReceivedNovember10,1987;acceptedDecember31,1987.Manystudieshavedemo
8、nstratedthepresenceofalteredmitosis,withdefectsinchromatinstructureandnuclearchromatinstructureintranscriptionallyactiveregi
