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1、ResearchArticleReceived8January2010,Received8April2010,Accepted9April2010PublishedonlineinWileyOnlineLibrary:4June2010(wileyonlinelibrary.com)DOI10.1002/bmc.1467Metabolicanalysisofrhubarbextractbyratintestinalbacteriausingliquidchromatography–tandemmassspectrome
2、trya,ba,ba,ba,ba,bRuiSong,LeiXu,FengguoXu,HaijuanDong,YuanTiananda,bZunjianZhang*ABSTRACT:Throughinvestigationofthemetabolismofrhubarbextractbyratintestinalbacteria,atotalof14componentsinrhubarbextractwerefoundtobebiotransformed.Thesecomponentsincludedaloe-emodi
3、n-O-glucosides,emodin-O-glucosides,chrysophanol-O-glucosides,physcion-O-glucosidesandthecorrespondingaglycones.Rheinalsocouldbebiotransformedbyratintestinalbacteria.Twelvemajormetabolitesweredetectedintheincubationsample.UnderESItandemmassconditions,thesequentia
4、lfragmentationpatternsof[M–H]-ionsweresimilartothoseoffreeanthraquinones,thusallowingtherapididentificationofthemetabolitesformedinincubationsamples.Theresultssuggestedthattheproposedhydrolysisofglycosidegroupfollowedbyhydrogenationinquinoidmoietyand/orfurtherace
5、tylationwasthemajorbiotrans-formationpathwayfortheseanthraquinoneglycosidesbyratintestinalbacteria.Copyright©2010JohnWiley&Sons,Ltd.Keywords:anthraquinoneglycosides;intestinalbacteria;metabolites;LC-DAD;LC-ESI-MS/MSIntroductionC,nearlyequimolaramountsofaloe-emod
6、inanthroneandrhei-nanthroneweredetectedinthelargeintestineofmice(Yamau-Rhubarb(Rheirhizoma)isoneoftheimportantherbaldrugsusedchietal.,1992).Rhein,rhein-8-O-glucoside,aloe-emodinandinorientalmedicine,andhaspurgative,choleretic,liverprotect-chrysophanolcouldbetran
7、sformedbyhumanintestinaling,antibacterial,anti-inflammatoryandanticarcinogeniceffectsbacteroidespeciesRHEIN-IandRHEIN-II(deWitteandLemli,(Duke,2002;JiaoandDu,2000;Xiaoetal.,1984)andcontains1988;deWitteetal.,1992;Meselhyetal.,2001).However,allthemanycompounds(Jiaoa
8、ndDu,2000;Kashiwadaetal.,1986a,b,abovereportsofthemetabolicanalysiswereconcernedonly1989;Linetal.,2006;Nonakaetal;1977,1981;Yeetal.,2007),withseveralpurecompoundsisol
