体外消化实验

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1、体外模拟消化（口腔、胃、小肠）：1、参考Gawlik-Dziki等（2009）方法（TheeffectofsimulateddigestioninvitroonbioactivityofwheatbreadwithTartarybuckwheatflavonesaddition）2.4.1.Preparationofsimulatedsaliva,gastricandintestinalfluidsSimulatedsalivasolutionwaspreparedbydissolveof2.38gNa2HPO4,0.19gKH2PO4

2、and8gNaClin1lofdistilledwater.ThesolutionwasadjustedtopH6.75anda-amylase(EC3.2.1.1.)wasaddedtoobtain200Uofenzymeactivity.Forthegastricdigestion,0.32%pepsin(fromporcinestomachmucosa,pepsinA,EC3.4.23.1)dilutionin0.03MNaCl,pH1.2wasprepared.Simulatedintestinal.juicewasprepar

3、edbydilutionof0.05gofpancreatinand0.3gofbileextractin35ml0.1MNaHCO3.2.4.1.Preparationofsimulatedsaliva,gastricandintestinalfluidsSimulatedsalivasolutionwaspreparedbydissolveof2.38gNa2HPO4,0.19gKH2PO4and8gNaClin1lofdistilledwater.ThesolutionwasadjustedtopH6.75andα-amylase

4、(EC3.2.1.1.)wasaddedtoobtain200Uofenzymeactivity.Forthegastricdigestion,0.32%pepsin(fromporcinestomachmucosa,pepsinA,EC3.4.23.1)dilutionin0.03MNaCl,pH1.2wasprepared.Simulatedintestinaljuicewaspreparedbydilutionof0.05gofpancreatinand0.3gofbileextractin35ml0.1MNaHCO3.1、参考V

5、ersantvoort等（2005）（Applicabilityofaninvitrodigestionmodelinassessingthebioaccessibilityofmycotoxinsfromfood）和Hur等（2009）的方法（Influenceofinitialemulsifiertypeonmicrostructuralchangesoccurringinemulsifiedlipidsduringinvitrodigestion）Table1Constituentsandconcentrationsoftheva

6、rioussyntheticjuicesoftheinvitrodigestionmodelrepresentingfedconditionsSaliva唾液Gastricjuice胃液Duodenaljuice十二指肠液Bilejuice胆汁Inorganicsolution10mlKCl89.6g/l15.7mlNaCl175.3g/l40mlNaCl175.3g/l30mlNaCl175.3g/l10mlKSCN20g/l3.0mlNaH2PO488.8g/l40mlNaHCO384.7g/l68.3mlNaHCO384.7g/l

7、10mlNaH2PO488.8g/l9.2mlKCl89.6g/l10mlKH2PO48g/l4.2mlKCl89.6g/l10mlNaSO457g/l18mlCaCl2·2H2O22.2g/l6.3mlKCl89.6g/l150μlHCl37%g/g1.7mlNaCl175.3g/l10mlNH4Cl30.6g/l10mlMgCl25g/l20mlNaHCO384.7g/l6.5mlHCl37%g/g180μlHCl37%g/gOrganicsolution8mlurea25g/l10mlglucose65g/l4mlurea25g/

8、l10mlurea25g/l10mlglucuronicacid(葡萄糖醛酸)2g/l3.4mlurea25g/l10mlglucosaminehydrochloride(盐酸氨基葡萄糖)33g/lAddt