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1、AminoAcidsDOI10.1007/s00726-010-0680-6REVIEWARTICLE100%proteinsequencecoverage:amodernformofsurrealisminproteomicsBjoernMeyer•DimitriosG.Papasotiriou•MichaelKarasReceived:19March2010/Accepted:25June2010ÓSpringer-Verlag2010AbstractThisreviewintendsnotonlytodiscusstheProloguecurrentp
2、ossibilitiestogain100%sequencecoverageforproteins,butalsotopointoutthecriticallimitstosuchanInitially,itisnecessarynotonlytopresenttheimportanceattempt.Theaimof100%sequencecoverage,astheofahighor100%proteinsequencecoverage,butalsothereviewtitlealreadyimplies,seemstoberathersurreali
3、fconstraintsthathindertherealization.Itcouldevenbesaidthecomplexityanddynamicrangeofaproteomeistakenthattheideafor100%sequencecoverageiscomparabletointoconsideration.Nevertheless,establishedbottom-upthewayofthinkinginsurrealism.Nevertheless,proteomicsshotgunapproachesareabletorough
4、lyidentifyacompletepioneerM.R.Wilkinsalreadypointedoutinthebeginningproteomeasexemplaryshownbyyeast.However,thisoftheproteomicserathat100%proteinidentificationisproceedingignoresmoreorlessthefactthataproteinisworthwhile(Wilkinsetal.1996).Thequestioniswhypresentasvariousproteinspecie
5、s.Theunambiguouseveryproteinshouldbeidentifiedwith100%sequenceidentificationofproteinspeciesrequires100%sequencecoverageandwhyallthisshouldbe‘‘beyondtherealism’’,coverage.Furthermore,theseparationoftheproteomeinotherwords,‘‘surrealism’’.mustbeperformedontheproteinspeciesandnotontheIn
6、AnnoDomini1994,Wilkinsdefinedtheproteomicspeptidelevel.Therefore,top-downisagoodstrategyforconcept(Wasingeretal.1995).Accordingtohim,theproteinspeciesanalysis.Classical2D-electrophoresisfol-proteomecanbedescribedasthecompleteproteinmapoflowedbyanenzymaticorchemicalcleavage,whichisat
7、hegenomeofanorganism,acelloracellularcompart-combinationoftop-downandbottom-up,isanotherinter-ment.Morethanadecadelater,thecompleteproteomeestingapproach.Moreover,thereviewsummarizesfurtheranalysisseemstobefeasible.In2006,Mannetal.weretop-downandbottom-upcombinationsandtowhichexten
8、tabletoshowthathalfoftheap
