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1、perspectiveProteomics:apragmaticperspectiveParagMallick1,2&BernhardKuster3,4Theevolutionofmassspectrometry–basedproteomichurdlesthanencounteredforeithergenomeortranscriptomestud-technologieshasadvancedourunderstandingofthecomplexies.Inparticular,issuesrelatedtos
2、plicevariants,post-translationalanddynamicnatureofproteomeswhileconcurrentlyrevealingmodifications(PTMs),dynamicranges(SupplementaryGlossary)thatno‘one-size-fits-all’proteomicstrategycanbeusedtoofcopynumbersspanningtenordersofmagnitude,proteinstabil-addressallbi
3、ologicalquestions.Whereassometechniques,ity,transientproteinassociationsanddependenceoncelltypeorsuchasthoseforanalyzingproteincomplexes,havematuredphysiologicalstatehavelimitedourtechnicalabilitytocharacterizeandarebroadlyappliedwithgreatsuccess,others,suchaspr
4、oteomescomprehensivelyandreproduciblyinareasonabletime5.globalquantitativeproteinexpressionprofilingforbiomarkerDespitethehurdles,after15yearsofevolution,proteomictechnolo-discovery,arestillconfinedtoafewexpertlaboratories.gieshavesignificantlyaffectedthelifesci
5、encesandareanintegralpartInthisPerspective,weattempttodistillthewidearrayofofbiologicalresearchendeavors(SupplementaryFig.1).conceivableproteomicapproachesintoacompactcanonofAtpresent,thefieldofproteomicsspansdiverseresearchtop-techniquessuitedtoaskingandansweri
6、ngspecifictypesofics,rangingfromproteinexpressionprofilingtoanalyzingsignal-biologicalquestions.Bydiscussingtherelationshipbetweeningpathwaystodevelopingproteinbiomarkerassaysystems.Itisthecomplexityofabiologicalsampleandthedifficultyofimportanttonotethatwithine
7、acharea,distinctscientificquestionsimplementingtheappropriateanalysisapproach,wecontrastarebeingaskedand,therefore,distinctproteomicapproachesmayareasofproteomicsbroadlyusabletodaywiththosethathavetobeapplied;theseapproachesvarywidelyintheirversatility,requiresi
8、gnificanttechnicalandconceptualdevelopment.technicalmaturity,difficultyandexpense.Consequently,wemustWehopetoprovidenonexpertswithaguideforcalibratingrecognizethatsom
