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1、MALDI样品制备方法样品制备流程TheidealsamplepreparationinMALDIwouldbeahomogenouslayerofsmallmatrixcrystalscontainingasolidsolutionoftheanalyte.待分析物种类/分析目的选择适当的基质选择适当的样品靶选择适当的点靶方法配置样品/基质溶液点靶命名法Targettypes:GroundSteelPolishedSteelAnchorChipStandard(800µm)AnchorChipSmall(400µm)Mat
2、rices&HCCAα-Cyano-4-hydroxycinnamicacid(BrukerPart-No.#201344)additives:2,5-DHB2,5-Dihydroxybenzoicacid(BrukerPartno.#201346)sDHB90:10mixtureof2,5-DHBand2-Hydroxy-5-methoxybenzoicacid(BrukerPartno.#209813)2,5-DHAP2,5-Dihydroxyactetophenone(BrukerPartNo.#231829)SATr
3、ans-3,5-dimethoxy-4-hydroxycinnamicacid(BrukerPartNo.#201345)1,5-DAN1,5-Diaminonaphthalene3-HPA3-Hydroxypicolinicacid(BrukerPartNo.#201224)DACDiammoniumhydrogencitrateNH4H2PO4Ammoniumphosphate,monobasicTFATrifluoroaceticacidSolvents:ACNAcetonitrileTA3030:70(v/v)Ace
4、tonitrile:TFA0.1%inwaterTA5050:50(v/v)Acetonitrile:TFA0.1%inwaterTA8585:15(v/v)Acetonitrile:TFA0.1%inwaterTA9090:10(v/v)Acetonitrile:TFA0.1%inwaterTA9595:5(v/v)Acetonitrile:TFA0.1%inwaterEtOHEthanolH2OWater一、基质的选择AnalyteMatrix多肽HCCAα-Cyano-4-hydroxycinnamicacid多肽/多
5、糖/蛋白2,5-DHB2,5-Dihydroxybenzoicacid2,5-DHAP2,5-Dihydroxyactetophenone蛋白SASinapicacidsDHB90:10mixtureof2,5-DHBand2-Hydroxy-5-蛋白（TDS）methoxybenzoicacid1,5-DAN1,5-Diaminonaphthalene核酸3-HPA3-HydroxypicolinicacidIAAtrans-Indole-3-acrylicacid聚合物DITDithranol二、样品制备法的选择•Dri
6、eddropletmethod•基质溶液与样品溶液等体积混合，直接点靶•Thinlayermethod•先点基质形成薄层，再点样品•Doublelayermethod•先点基质，再按照drieddroplet法点样三、靶板的选择常规样品分析。灵敏度一般Groundsteeltarget常规样品分析。灵敏度一般PolishedsteeltargetAnchorChipStandard(800µm)蛋白质组学样品(酶解样品)，灵敏度较好AnchorChipSmall(400µm)适用于寡核苷酸样品分析GroundSteel•适用
7、范围•所有基质的drieddroplet样品制备法•聚合物分析•SA基质doublelayer样品制备法•优点•对污染物的容忍度比较好•缺点•相对不适合于自动化采集•灵敏度较低Polishedsteel•适用范围•与groundsteel一样，应用范围广•最适用于thinlayer样品制备法•优点•有机溶剂扩散快，有利于形成薄层•高分子量聚合物分析（>100kDa）•缺点•采用drieddroplet样品制备法，易形成较大的晶体颗粒，质量精度比较差•采用thinlayer样品制备法，采集数据时，需要不停移动采样位置（rand
8、omwalk）ProtocolOverviewAnalyteMatrixTargetTypeProtocol多肽HCCAGroundSteelDrieddropletAnchorChipStandard(800µm)DrieddropletAnchorChipStandard(800µm)
